糖尿病大鼠阴茎的氧化应激与阴茎勃起功能障碍的关系  

Effect of oxidative stress on diabetic erectile dysfunction in rats

作　　者：刘丽[1] 王文进[2] 朱浩[2] 李志宏[1] 丁文龙[2] 

机构地区：[1]上海健康职业技术学院解剖学教研室,上海200237 [2]上海交通大学医学院解剖学教研室,上海200025

出　　处：《解剖学报》2010年第6期872-875,共4页Acta Anatomica Sinica

基　　金：上海市科技发展基金资助项目(03ZR14042);上海市重点学科建设资助项目(S30201)

摘　　要：目的探讨氧化应激在糖尿病性阴茎勃起功能障碍(ED)中的作用。方法注射链脲佐菌素建立糖尿病大鼠模型,分别在注射8周和12周后观察阴茎勃起次数;取大鼠阴茎,测定阴茎丙二醛水平、总抗氧化能力,免疫印迹法检测阴茎核因子E2相关因子2(Nrf2)蛋白量的变化。结果糖尿病大鼠的阴茎勃起次数明显低于对照组,并随病程延长降低;糖尿病大鼠阴茎丙二醛水平明显高于对照组,总抗氧化能力水平明显低于对照组;与对照组比较,糖尿病组大鼠阴茎内Nrf2蛋白量随病程延长而降低。结论糖尿病性阴茎勃起功能障碍与阴茎氧化应激水平升高相关。Objective To investigate the effect of oxidative stress on diabetic erectile dysfunction （ ED ）. Methods Diabetes mellitus（DM） was induced by streptozotocin （STZ） injection. Eight weeks and twelve weeks later, penis erectile function was detected by injecting apomorphine. The concentrations of malondialdehyde （MDA） and total anti-oxygen capability （T-AOC） in penis were measured. Western blotting was used to measure nuclear factor E2-related factor 2（Nrf2） in both control and diabetic rat penis tissues in present study. Results Compared with control group, the erection times of DM rats decreased significantly. The mean level of MDA in penis of DM rats were higher than that of control group（P 〈 0. 01） , and the mean level of T-AOC in DM rats was lower than that of control（P 〈 0.05）. Nrf2 protein expression decreased in DM groups compared with that of control（P 〈 0.05）. Conclusion Oxidative stress is probably involved in the mechanism inducing diabetic ED.

关 键 词：糖尿病 阴茎勃起功能障碍 丙二醛 总抗氧化能力 核因子E2相关因子2 硫代巴比妥酸比色法 免疫印迹法 大鼠 

分 类 号：R34[医药卫生—基础医学]