纳米银粒子表面增强拉曼光谱在宠物饲料中三聚氰胺的速测研究  

Rapid Determination of Melamine in Pet Food by Surface Enhanced Raman Spectroscopy in Combination with Ag Nanoparticles

作　　者：程劼[1,2] 苏晓鸥[1,2] 

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所 [2]国家饲料质量监督检验中心(北京),北京100081

出　　处：《光谱学与光谱分析》2011年第1期131-135,共5页Spectroscopy and Spectral Analysis

基　　金：国家"十一五"科技支撑计划项目(2006BAD12B03)资助

摘　　要：研究将制备的纳米银粒子作为表面增强拉曼光谱的增强试剂,实现对宠物饲料中三聚氰胺的快速定性定量分析。以709与1542cm-1处拉曼位移作为定性依据,以1149cm-1峰强度作为归一化标准,在1.0～10.0mg·kg-1浓度范围实现定量计算,检测限0.5mg·kg-1。研究发现,纳米银粒子对三聚氰胺具有较强的拉曼增强效应,特征峰信号强度与纳米银粒子加入时间,溶液涡旋强度有很大的关系,同时也受提取溶剂的种类、提取方式及样品加入量的影响。本方法中每个样品5min内完成分析,与现有方法相比,快速简便,对宠物饲料中三聚氰胺实现现场、快速检测。The rapid qualitative and quantitative analysis of melamine in pet food was realized by surface-enhanced Raman spectroscopy in combination with Ag nanoparticle.In the present study,the 709 and 1 542 cm-1 Raman shift was chosen as qualitative basis.The quantitative calculation of the concentration range between 1.0 and 10.0 mg·kg-1 was achieved based on the intensity of 1 149 cm-1 Raman peak which was used as a normalization standard.The limit of detection was 0.5 mg·kg-1.The Ag nanoparticle had a strong Raman enhancement effect on melamine and the intensity was affected by the adding time of Ag nanoparticle and the vortex strength.At the same time,the intensity of SERS was affected by the extraction solvent type,and the manner of extracton.The analysis time of each sample was about 5 min.It was so quick that it was easy to realize the rapid detection of melamine in pet food compared with existing methods.

关 键 词：表面增强拉曼光谱 纳米银粒子 三聚氰胺 宠物饲料 快速定性定量分析 

分 类 号：O657.3[理学—分析化学]