磷酸铵镁结石患者尿微晶组分分析及其与结石形成的关系  

Composition Analyses of Urinary Microcrystalline in Urine of Magnesium Ammonium Phosphate Stones Formers and Its Relationship with the Stones Formation

作　　者：杨锦[1] 黄志杰[1] 侯善华[1] 欧阳健明[1] 

机构地区：[1]暨南大学生物矿化与结石病防治研究所,广东广州510632

出　　处：《光谱学与光谱分析》2011年第1期168-172,共5页Spectroscopy and Spectral Analysis

基　　金：广东省科技攻关项目(2009B030801236);国家自然科学基金项目(30672103)资助

摘　　要：采用X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)、纳米粒度仪、扫描电子显微镜(SEM)和透射电子显微镜(TEM)研究了磷酸铵镁结石患者尿液中微晶的组分、形貌、粒径和Zeta电位,并对其结石进行了组分分析。结果表明,结石类型、尿微晶组分和尿液pH三者之间存在密切的联系:磷酸铵镁结石病人的尿液pH值较高,通常在6.5以上;尿微晶的主要组分是含不同结晶水(如一水和六水)的磷酸铵镁晶体;磷酸铵镁晶体主要为花瓣形、十字花形,微晶的粒度分布不均匀,分布范围宽,并且发生明显聚集。磷酸铵镁结石患者的Zeta电位负值[平均值(-9.83±0.66)mV]与健康对照者的[平均值(-10.74±0.25)mV]没有明显差异。此研究有助于预测尿石症的发生,并为预测尿石的类型提供启示。By means of X-ray diffraction（XRD）,Fourier transform infrared spectroscopy（FTIR）,nano-particle size analyzer,scanning electron microscopy（SEM） and transmission electron microscopy（TEM）,the composition,morphology,particle size and zeta potential of urinary microcrystalline in urine of magnesium ammonium phosphate stone formers were investigated.The components of stones were also analyzed.The results showed that there was a close relationship among stone components,urinary microcrystalline composition and urine pH.A high pH value of 6.5 or more usually appeared in the urine of magnesium ammonium phosphate stone formers.The main component of urine microcrystalline was magnesium ammonium phosphate crystals with different crystal water such as monohydrate or hexahydrate.Magnesium ammonium phosphate crystals are mainly petal-shaped,crosswise shape.These microcrystalline have an uneven particle size distribution,a wider distribution range,and apparent aggregation.There is no significant difference in the zeta potential between the magnesium ammonium phosphate stone formers（mean（-9.83±0.66） mV） and healthy control subjects（mean（-10.74±0.25） mV）.This study can help predict the occurrence of urolithiasis,and provide inspiration to the prediction of the type of urinary stones.

关 键 词：磷酸铵镁 结石 尿微晶 ZETA电位 

分 类 号：O434[机械工程—光学工程] O611.6[理学—光学]