土贝母皂甙对单纯疱疹病毒性角膜炎作用的实验研究  

Experimental research on the efficacy of tubeimoside on herpes simplex keratitis

作　　者：张晓辉[1] 王建明[1] 孙乃学[1] 

机构地区：[1]西安交通大学医学院第二附属医院眼科,中国陕西省西安市710004

出　　处：《国际眼科杂志》2011年第1期22-24,共3页International Eye Science

摘　　要：目的:探讨土贝母皂甙(tubeimoside,Tu)对兔单纯疱疹病毒性角膜炎(herpes simplex keratitis,HSK)作用的效果。方法:选取24只兔建立HSK模型,随机分4组,即生理盐水、阿昔洛韦、Tu和Tu联合应用重组牛碱性成纤维细胞生长因子(recombinant bovine basic fibroblast growth factor,bFGF)。裂隙灯显微镜、扫描电镜和透射电镜观察眼部病变的动态变化。8只兔左眼滴0.4g/L Tu,右眼滴生理盐水。结果:Tu、阿昔洛韦、Tu+bFGF与生理盐水4组间比较,角膜上皮病变分值(corneal epithelial involvement scores,CEIS)均有显著性差异(q=7.75,20.50,22.17,P<0.01)。阿昔洛韦组和Tu组、Tu+bFGF组相比,CEIS差异有显著性(t=2.1,1.9,P<0.05)。Tu组与Tu+bFGF相比,CEIS有显著性差异(t=2.17,P<0.05)。结论:采用0.4g/L Tu治疗实验性HSK有一定的疗效,联合应用bFGF可以增强Tu治疗HSK的疗效。0.4g/L Tu滴眼有轻度刺激性。AIM： To evaluate the efficacy of tubeimoside （Tu） on herpes simplex keratitis （HSK） of rabbits. METHODS： HSK models were established in 24 rabbits, which were randomly divided into 4 groups： normal saline （NS）, acyclovir （ACV）, Tu and Tu combined with recombinant bovine basic fibroblast growth factor （bFGF）. All eyes were examined and corneal samples lesions dynamic changes were selectively observed by slit-lamp microscope, scanning and transmission electron microscope. RESULTS： Among 4 groups of NS, ACV, Tu and Tu ＋bFGF, corneal epithelial involvement scores （CEIS） showed significant difference （P〈0.01）;compared ACV with Tu or Tu ＋bFGF respectively, CEIS had significant difference （P〈0.05）, as well as that between Tu and Tu ＋bFGF （P〈0.05）. CONCLUSION： There is curative effect on HSK with Tu（0.4g/L）, and the therapeutic effect can be enhanced when combined with bFGF except for slight stimulatory.

关 键 词：土贝母皂甙 单纯疱疹病毒 角膜炎 

分 类 号：R285.5[医药卫生—中药学]