慢性乙型肝炎患者YMDD自然变异的检测  

DETECTION OF HBV YMDD MUTATION IN PATIENTS WITH CHRONIC HEPATITIS B BEFORE ANTI-VIRUS TREATMENT

作　　者：田珊红[1] 胡金华[2] 

机构地区：[1]河北省唐山市传染病医院,唐山063000 [2]河北省唐山市华北煤炭医学院

出　　处：《现代预防医学》2011年第1期140-141,147,共3页Modern Preventive Medicine

摘　　要：[目的]检测未接受抗病毒治疗的慢性乙型肝炎患者HBV基因型及其YMDD变异的情况。[方法]选择慢性乙型肝炎患者中未接受抗病毒治疗的54例为研究对象,应用PCR荧光分子信标探针检测技术及PCR微板核酸杂交-ELISA技术进行HBV YMDD变异株及HBV基因分型检测。[结果]①54例未接受抗病毒治疗的慢性乙型肝炎患者HBV基因型以C、B、D亚型多见,分别占44.4%(24/54),22.2%(12/54),14.8%(8/54)。其中检测出YMDD变异阳性10例,变异率为18.5%(10/54);不同基因型YMDD变异率差异无统计学意义。②YMDD变异者与未变异者肝脏转氨酶检测值比较差异无统计学意义[ALT为(276.91±78.23)U/L比(255.72±70.20)U/L,AST为(237.44±73.25)U/L比(248.76±69.08)U/L,P均﹥0.05]。[结论]唐山地区慢性乙型肝炎患者HBV基因型以C、B、D亚型多见,并存在天然YMDD变异株;发生YMDD变异者肝脏损害未加重。[Objective] To investigate the existence of HBV YMDD mutation and their genotypes in patients with chronic hepatitis B before anti-virus treatment. [Methods] HBV YMDD mutation was detected in 54 patients with chronic hepatitis B by molecular beacon fluorescent probes, and the genotypes were detected by the method of polymerase chain reaction （PCR） microplate hybridization ELISA. [ Results] ① HBV DNA YMDD mutation was found in 10 out of 54 patients with hepatitis B （positive rate 18.5%）. Among 54 patients, 44.4% was genotype C, 22.2% was genotype B, and 14.8% was genotype D. The mutation rates of deferent genotypes showed no significant deference. ② There was no significant deference in ALT （276.91± 78.23U/L vs 255.72±70.20U/L, P 〉 0.05） and AST （237.44±73.25U/L vs 248.76±69.08U/L, P 〉 0.05） between the eases with YMDD mutation and the ones without mutation. [Conclusion] In Tangshan city, the genotypes of chronic hepatitis B patients are mainly C, B, D. Among them, HBV DNA YMDD mutation may exist naturally, but the YMDD mutation cases does not show heavier heptie function damage.

关 键 词：慢性乙型肝炎 YMDD变异 基因型 

分 类 号：R512.62[医药卫生—内科学]