2009甲型H1N1亚型流感病毒血凝素重链(HA1)基因分析  

GENETIC ANALYSIS ON HA1 GENE OF 2009 INFLUENZA A(H1N1)VIRUSES

作　　者：龚甜[1] 熊英[1] 刘丽萍[1] 王飞霞[1] 施勇[1] 周珺[1] 

机构地区：[1]江西省疾病预防控制中心,南昌330029

出　　处：《现代预防医学》2011年第1期146-147,共2页Modern Preventive Medicine

摘　　要：[目的]从基因层面初步探讨近8个月甲型H1N1流感病毒HA1基因变异情况。[方法]收集19株2009年5～12月欧美及亚洲最新上传的甲型H1N1流感流行株血凝素重链(HA1)区域的氨基酸序列,分析其与墨西哥城第一株流感病毒分离株A/MexicoCity/001/2009在HAl以及HAl抗原决定簇区域的氨基酸差异。[结果]与A/Mex-icoCity/001/2009比较,HA1区氨基酸序列的同源性为96.98%～99.7%;并且,A/Pennsylvania/09/2009、A/Fukuoka-C/1/2009和A/Milan/UHSR1/20093株流感流行株HA1区抗原决定簇B区197位氨基酸出现了A﹥T替换。[结论]2009甲型H1N1流感流行的近8个月HA1基因存在一定的变异。[Objective ] To preliminary study the genetic variation of influenza A （H1N1） viruses HA1 in last 8 months. [ Methods] Collected the amino acid sequence of influenza hemagglutinin heavy chain （HA1） of 19 most recently uploaded influenza A （H1N1） pandemic strains in Europe, America and Asia from May to November in 2009, analyzed the amino acid sequence differences in the HAl and HA1 epitope amino acid of the Mexico City firstly isolated influenza virus strains （A/MexicoCity/O01/2009）. [Results] Compared with the A/MexicoCity/001/2009, the homology was 96.98%-99.7% in amino acid sequence of HA1; and 197 amino acids of HA1 epitope amino acid B appealed A〉 T replacement in 3 influenza virus strains, which were A/Pennsylvania/09/20091 A/Fukuoka--C/l/2009and A/Milan/UHSR1/2009. [ Conclusion] There is a certain variation of HA1 gene in 2009 influenza A （H1N1） pandemic in the last 8 months.

关 键 词：流感病毒 2009甲型H1N1亚型 HA1基因 

分 类 号：R373.13[医药卫生—病原生物学]