Analysis on the DNA Fingerprinting of Aspergillus Oryzae Mutant Induced by High Hydrostatic Pressure  

作　　者：WANG Hua ZHANG Jian YANG Fan WANG Kai SHEN Si-Le LIU Bing-Bing ZOU Bo ZOU Guang-Tian 

机构地区：[1]State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012 [2]College of Life Science, Jilin University, Changchun 130012

出　　处：《Chinese Physics Letters》2011年第1期54-57,共4页中国物理快报（英文版）

基　　金：Supported by the National Natural Science Foundation of China under Grant Nos 20773043, 21073071 and 10979001, and the National Basic Research Program of China under Grant Nos 2005CB724400 and 2007CB808000.

摘　　要：The mutant strains of aspergillus oryzae （HP300a） are screened under 300 MPa for 20min. Compared with the control strains, the screened mutant strains have unique properties such as genetic stability, rapid growth, lots of spores, and high protease activity. Random amplified polymorphic DNA （RAPD） and inter simple sequence repeats （ISSR） are used to analyze the DNA fingerprinting of HP300a and the control strains. There are 67.9% and 51.3% polymorphic bands obtained by these two markers, respectively, indicating significant genetic variations between HP300a and the control strains. In addition, comparison of HP300a arid the control strains, the genetic distances of random sequence and simple sequence repeat of DNA are 0.51 and 0.34, respectively.The mutant strains of aspergillus oryzae （HP300a） are screened under 300 MPa for 20min. Compared with the control strains, the screened mutant strains have unique properties such as genetic stability, rapid growth, lots of spores, and high protease activity. Random amplified polymorphic DNA （RAPD） and inter simple sequence repeats （ISSR） are used to analyze the DNA fingerprinting of HP300a and the control strains. There are 67.9% and 51.3% polymorphic bands obtained by these two markers, respectively, indicating significant genetic variations between HP300a and the control strains. In addition, comparison of HP300a arid the control strains, the genetic distances of random sequence and simple sequence repeat of DNA are 0.51 and 0.34, respectively.

关 键 词：Instrumentation and measurement Medical physics Biological physics 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] TQ920.1[农业科学—植物保护]