Box-Behnken效应面法优化白芍配方颗粒提取工艺  

Optimization of Processing Parameters for Extraction of Total Glucoside of Paeony in Paeony Dispensing Granule Using Box-Behnken Experimental Design

作　　者：柳俊[1,2] 张建军[2] 

机构地区：[1]广州中医药大学,广州510405 [2]广东省中医研究所,广州5100952

出　　处：《中国实验方剂学杂志》2011年第1期9-13,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选白芍配方颗粒中白芍总苷提取的工艺条件。方法:采用单因素试验结合Box-Behnken效应面法,以白芍总苷提取量为指标进行试验,考察加水量、提取时间、提取温度、提取次数、浸泡时间等因素对提取工艺的影响。结果:优选出水提白芍总苷的最佳工艺为:浸泡98.4 min,加12倍量水,煎煮提取2次,每次69.8 min。白芍总苷的提取率为2.387 1%。结论:Box-Behnken试验设计法用于白芍配方颗粒提取工艺的优选是可行的,数学模型的预测值与试验观察值相符。Objective：Optimization of the processing parameters was applied for the extraction of total glucoside of paeony in paeony dispensing granule. Method： Based on the results of single-factor-at-a-time-technique, Box-Behnken experimental design combining with response surface methodology（RSM） was employed, with the soaking time （X1）, the water addition （X2 ）, the extraction time （X3 ） as the independent variables. The response variable was the amount of total glucoside of paeony. Result： The optimization processing parameters was as follows ： X1, X2 and X3 levels of 98.4 min, 12 times and 69.8 min respectively. Conclusion：The observed responses were in close agreement with the predicted values of the mathematic models, and the Box-Behnken experimental design is suitable for optimizing the extraction of total glucoside of paeony in paeony dispensing granule.

关 键 词：白芍配方颗粒 白芍总苷 提取工艺 Box—Behnken效应面法 

分 类 号：R283.6[医药卫生—中药学]