巴戟天炮制前后蒽醌类成分含量变化  

Simultaneous Analysis of Anthraquinones in Processed Pieces and Crude Drugs from Morinda officinalis

作　　者：姜永粮[1] 杨丹[1] 张凡[1] 史辑[1,2] 贾天柱[1,2] 

机构地区：[1]辽宁中医药大学,辽宁大连116600 [2]辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中国实验方剂学杂志》2011年第1期64-66,69,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:对巴戟天炮制前后3种蒽醌类化学成分的含量进行比较研究。方法:采用HPLC同时测定3种蒽醌类成分1,8-二羟基-3-羟甲基蒽醌,1,8-二羟基-3-甲基-5-羟甲基蒽醌,1-甲基蒽醌的含量,色谱柱:Agilent-C18(4.6 mm×150 mm,5μm);流动相以甲醇-水梯度洗脱(60∶40→85∶15,25 min);流速1.00 mL·min-1;柱温30℃;检测波长278 nm;进样量10μL。测定巴戟天生品和炮制品中三种蒽醌的含量。结果:3种蒽醌的线性范围分别为1,8-二羟基-3-羟甲基蒽0.019 9～0.199 2μg,1,8-二羟基-3-甲基-5-羟甲基蒽醌0.0331～0.331 2μg,1-甲基蒽醌0.0197～0.196 8μg,3种蒽醌的平均回收率均在98.50%～100.31%。巴戟天炮制前后蒽醌类成分有所变化。结论:所建立的分析方法可以同时测定巴戟天不同炮制品3种蒽醌类化合物的含量,为解析巴戟天炮制原理提供一定的科学依据。Objective：To compare the content of anthraquinones in the different processed products from Morinda officinalis How. Method： 1-methylanthraquinone, 1,8-dihydroxy-3-methyl-5-hydroxymethyl-anthraquinone, 1,8-dihydroxy-3-hydroxymethyl-anthraquinone were determined simultaneously by HPLC. The separation was performed on a Agilent C18 column at 30℃ with MeOH-H20 （60：40→85：15, 25 rain） as a mobile phase. The detection wavelength was set at 278 nm and the flow rate was 1.00 mL·min^-1. Result： Three components were linear within the range of 0. 019 9-0. 199 2μg, 0. 0331-0. 3312μg, 0. 019 7-0. 196 8μg, and the average recoveries were 98.50%-100.31% respectively. Conclusion： The method can be used for evaluating the quality in different processed pieces of M. officinalis.

关 键 词：巴戟天 1-甲基蒽醌 1 8-二羟基-3-甲基-5-羟甲基蒽醌 1 8-二羟基-3-羟甲基蒽醌 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]