四逆散对实验性溃疡性结肠炎大鼠IL-1β的影响  

Study on Influence of Sini Powders on IL-1β in Rats with Experimental Ulcerative Colitis

作　　者：卢健[1,2] 马骥[1] 王丽娜[1] 林蔗茹[1] 于彩娜[1] 

机构地区：[1]辽宁中医药大学方剂学科,沈阳110032 [2]辽宁卫生职业技术学院,沈阳110101

出　　处：《中国实验方剂学杂志》2011年第1期103-105,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅项目(2006T093)

摘　　要：目的:探讨四逆散对实验性溃疡性结肠炎(UC)大鼠白介素-1β(IL-1β)的影响,以及君臣配伍在组方中的作用。方法:将实验动物分为5组,采用免疫法造模,以大鼠结肠组织大体情况、结肠组织病理变化和IL-1β水平作为观测指标,用ELISA法检测大鼠结肠黏膜组织和血清IL-1β水平。结果:模型组大鼠结肠组织黏膜层可见溃疡形成,且有明显的炎症存在,认为本实验造模成功;模型组大鼠血清和结肠黏膜组织的IL-1β分别为(841.93±95.64)ng·L-1和(75.06±2.15)ng·L-1均显著高于正常组(P<0.01),灌服柴芍枳(1.31 g·kg-1)和柴芍(0.88 g·kg-1)后大鼠血清和结肠黏膜组织IL-1β显著降低(P<0.05),而灌服四逆散(1.75 g·kg-1)后大鼠结肠黏膜组织IL-1β(72.62±2.67)ng·L-1明显减少(P<0.01),但血清IL-1β(1015.52±176.47)ng·L-1却显著升高。结论:四逆散能够通过调节IL-1β来干预实验性UC,其全方发挥作用环节可能为抑制IL-1β由外周血单核细胞向炎症肠黏膜的移行;而方中君、臣药物配伍的作用环节为抑制IL-1β的分泌。Objective： To observe the influence of Sini Powders on interleukin-1 beta（IL-1β） of rats with experimental ulcerative colitis （UC） and effects of so-called the monarch drug and ministerial drug in the prescription. Method： The experimental animals were divided into 5 groups, and modeling was made by immunization. IL-1β levels in rats＇ colon mueosal tissues and serum were detected by ELISA. Result： Colon pathological changes with obvious inflammation were found after modeling. The levels of IL-1β in rats of the model group were higher than those in the normal group（P 〈 0.01 ）. After feeding Chaishaozhi and Chaishao, IL-1β levels of UC rats were obviously reduced （P 〈 0. 05）. After feeding Sini Powders, IL-1β levels in UC rats＇ colon mueosal tissue were markedly reduced （72.62± 2. 67）ng· L^-1, （P 〈 0. 01 ）, but they were obviously increased in serum （1015. 52± 176. 47）ng·L^-1. Conclusion： We speculate that Sini Powders can interfere with experimental UC by adjusting IL-1β levels. Sini Powders may play a role in inhibiting moving of IL-1β from the mononuclear cells in peripheral blood to intestinal mucosa, and the compatibility of the monarch drug and ministerial drug play a role in suppressing secretion of IL-1β.

关 键 词：四逆散 白介素1Β 溃疡性结肠炎 实验 配伍 

分 类 号：R285.5[医药卫生—中药学]