补肾中药对帕金森病模型小鼠黑质-纹状体多巴胺的影响  

Effects of Nourishing Kidney Herbs on Dopamine of Striatal Neurons in Mouse Model of Parkinson ’s Disease

作　　者：田允[1] 宋文婷[2] 徐立[2] 周凡[3] 蔡晶[1] 

机构地区：[1]福建中医药大学中西医结合研究院,福州350108 [2]中国中医科学院西苑医院,北京100091 [3]福建中医药研究院,福州350003

出　　处：《中国实验方剂学杂志》2011年第1期134-137,141,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：福建省自然基金杰青项目(2009J06018)

摘　　要：目的:探讨补肾中药对帕金森病(PD)模型小鼠黑质-纹状体神经递质多巴胺(DA)的影响。方法:取PD模型小鼠45只,随机分为淫羊藿组、女贞子组、黄精组、司来吉兰组和模型对照组,每组9只,另取同周龄C57BL/6J雄性小鼠9只作为正常对照组。正常对照组和模型对照组ig蒸馏水,淫羊藿组、女贞子组、黄精组、司来吉兰组分别ig淫羊藿、女贞子、黄精、司来吉兰(剂量分别为6,6,8,2×10-3 g·kg-1)的水煎液及水溶液。0.5 mL/次,2次/d,连续给药4周后冰浴中取黑质-纹状体,免疫组织化学法检测酪氨酸羟化酶(TH)的含量,高效液相-电化学法检测DA及其代谢物高香草酸(HVA)的含量。结果:淫羊藿组TH的表达,DA,二羟苯乙酸(DOPAC)的含量均高于模型对照组(P<0.05),且淫羊藿组TH的表达高于司来吉兰组(P<0.05),与空白对照组无差异;女贞子组和黄精组中DA的含量较模型对照组高(P<0.05),且女贞子组中DA的含量高于司来吉兰组(P<0.05),与空白对照组无差异。结论:补肾中药可以增加PD模型小鼠黑质-纹状体中DA的含量,但其作用机制并不完全一致,有待进一步深入研究。Objective： To explore the effects of nourishing kidney herbs on dopamine of striatal neurons in mouse model of parkinson ＇ s disease. Method ： Fourty five Parkinson＇ s disease model mice were randomly divided into five groups：Epimedii Folium group, Ligustri Lucidi Fructus group, Polygonatum group, selegiline group and model control group. Nine normal male C57BL/6J strains of mice were used as the normal control group. The normal control group and model control group were adminstraed with water, the mice in Epimedii Folium group, Ligustri Lucidi Fructus group, Polygonati Rhizoma group and selegiline group were adminstraed with Epimedii Folium, Ligustri Lucidum Fructus, Polygonatum and selegiline water decoction （each 0.5 mL, bid）. After for 4 weeks, striatal neurons were quickly separated in the ice. Immunohistochemistry was used to detect the level of TH, HPLC -Electrochemical was used to detect the level of DA and metabolites. Result ： The levels of TH, DA, DOPAC in Epimedii Folium group were significantly higher than that in model control group and selegiline group （P 〈 0.05） , were not significantly lower than that in the blank control group ;The levels of DA were significantly higher in Ligustri lucidi Fructus group and Polygonati Rhizoma group than that in the model group （P 〈 0.05 ）, while the levels in Ligustrum lucidum group was higher than selegiline group （P 〈 0.05 ） , but not higher than that in the blank control group. Conclusion： The nourishing kidney herbs Epimedium Folium, Ligustri Lucidi Fructus and Polygonati Rhizoma can improve DA of striatal neurons in mouse model of parkinson ＇ s disease, but the mechanism is not exactly the same. Further study is needed.

关 键 词：补肾中药 帕金森病 酪氨酸羟化酶 多巴胺 

分 类 号：R285.5[医药卫生—中药学]