柴胡加龙骨牡蛎汤有效部位抗抑郁作用机制研究  

Mechanism of Antidepressant-like Effect of Chaihu Jia Longgu Muli Decoction’s Active Fraction

作　　者：康大力[1] 瞿融[2] 朱维莉[3] 马世平[3] 

机构地区：[1]广东食品药品职业学院,广州510520 [2]南京中医药大学方剂教研室,南京210029 [3]中国药科大学中药药理教研室,南京210038

出　　处：《中国实验方剂学杂志》2011年第1期138-141,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省自然科学基金项目(BK2005149);江苏省中医药局中医药科研项目(H05074)

摘　　要：目的:研究柴胡加龙骨牡蛎汤有效部位(CLMAF)抗抑郁作用机制。方法:小鼠分为正常对照组,模型组,CLMAF组(20,40,80 mg·kg-1),阿米替林(20 mg.kg-1)组,连续ig 7 d通过测定强迫游泳小鼠脑内单胺氧化酶(MAO)、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量以及大鼠海马原代细胞培养初步探讨其抗抑郁作用机制。结果:CLMAF可显著抑制强迫游泳应激小鼠脑内MAO-A活力,并显著降低MDA含量,SOD活力也相应降低。同时,能够抵抗皮质酮(CORT)诱导的大鼠海马神经元损伤,使存活细胞数量增加。结论:CLMAF抗抑郁作用主要与抑制MAO-A活力,降低MDA含量和保护海马神经元等作用有关。Objective： To elucidate the mechanism of antidepressant-like effect of Chaihu Jia Longgu Muli Decoction＇ s active fraction （CLMAF）. Method： The activity of monoamine oxidase （MAO） and superoxide dismutase（SOD） and the content of malondialdehyde（MDA） in cerebral tissue were analyzed, and the protective effect for the rat hippocamp neurons was analyzed with MTT colorimetcry. Result： MAO-A activity, SOD activity and MDA content of forced swimming mice were inhibited and the lesion of rat hippocamp neurons induced by corticosterone was protected significantly. Conclusion： Mechanisms of antidepressant-like effect of CLMAF are related to inhibiting MAO-A activity and content of MDA and protecting hippocamp neurons.

关 键 词：柴胡加龙骨牡蛎汤 有效部位 抗抑郁 机制 

分 类 号：R285.5[医药卫生—中药学]