高良姜不同提取物对急性脑缺血小鼠脑血管通透性的影响  

Effect of Alpinia officinarum Extract on Brain Vascular Permeability in Mice

作　　者：赵燕燕[1,2] 刘新霞[3] 陈春生[2] 白洁[1] 

机构地区：[1]河北大学医学实验中心,河北保定071000 [2]河北大学化学与环境科学学院,河北保定071000 [3]河北大学医学部,河北保定071000

出　　处：《中国实验方剂学杂志》2011年第1期142-144,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省科技攻关项目(05276101D-88);河北教育厅科学研究计划项目(y2009315);河北省卫生厅医学科学研究重点课题计划项目(0501520090570);河北省中医药管理局科研计划课题(2008072)

摘　　要：目的:研究高良姜不同提取物对急性脑缺血小鼠脑血管通透性的影响。方法:昆明种小鼠随机分为9组:空白对照组、模型对照组、尼莫地平组、高良姜水提取物(WEAH)6.66,3.33,1.67 mg·kg-1组、高良姜乙醇提取物(EEAH)6.66,3.33,1.67 mg·kg-1组。连续ig 10 d后,采用双侧颈总动脉结扎法制备小鼠急性脑缺血模型,干湿重法观察脑含水量变化,通过收集透出脑血管外的伊文思蓝(Evans blue,EB)含量考察脑血管通透性的变化。结果:急性脑缺血发生后3 h,模型组小鼠与空白对照组相比较,脑含水量及脑组织内EB含量显著增加(P<0.05)。与模型组比较,尼莫地平组小鼠脑含水量及脑组织内EB含量均显著降低(P<0.05);高良姜水提取物6.66 mg·kg-1、乙醇提取物3.33 mg·kg-1及1.67 mg·kg-1组小鼠脑含水量显著降低(P<0.05);高良姜水提取物3个剂量组小鼠脑组织内EB含量均显著降低(P<0.05),乙醇提取物组小鼠脑组织内EB含量有降低趋势,但无显著性差异。结论:高良姜水及乙醇提物可有效降低急性脑缺血后脑含水量和脑血管的通透性,对脑血管有一定的保护作用。Objective： To observe the effect of Alpinia officinarum extract on blood vessel of brain permeability. Method ： Kunming mice were randomly divided into 9 groups （ control group, model group, nimodipine group, watere xtract of A. officinarum 6.66, 3.33, 1.67 mg·kg^-1 groups, Ethanol extract of A. officinarum 6. 66, 3.33, 1.67 mg·kg^-1 groups）. The mice model of total cerebral ischemia was established with method of bilateral common carotid artery occlusion after 10 days of ig treatment. The brain water content was evaluated by detecting the wet weight and dry weight of brain ; The detection of cerebral extra-vascular Evans blue （EB） content was used to observe the changes of cerebrovascular permeability. Result ： After acute cerebral ischemia for 3 hours, compared with the control group, brain water content and EB content of brain tissue increased significantly （P 〈 0.05） in the model control group mice. Compared with the model group, the brain water content and EB content of brain tissue were significantly decreased （P 〈 0.05） in nimodipine group ; the brain water content were significantly decreased （P 〈0. 05） in WEAH 6. 66 mg·kg^-1, EEAH 3.33 mg·kg^-1and 1.67 mg·kg^-1 ; brain tissue EB content were significantly decreased （P 〈 0.05） in three doses of aqueous extract groups. Conclusion： WEAH and EEAH can decrease the cerebral water content and eerebrovascular permeability effectively.

关 键 词：高良姜 脑血管通透性 干湿重法 伊文思蓝 

分 类 号：R285.5[医药卫生—中药学]