用微载体系统培养PK15细胞生产猪圆环病毒2型  

Production of PCV2 via PK15 cells cultured on microcarriers

作　　者：何锡忠[1] 李春华[1] 倪建平 蒋风英[1] 邹勇[1] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海佳牧生物制品有限公司,上海201106

出　　处：《上海农业学报》2010年第4期149-151,共3页Acta Agriculturae Shanghai

基　　金：上海市科委科技发展基金项目(04391930)资助

摘　　要：研究了生物反应器、转瓶、方瓶3种不同培养系统生产PCV2,对转瓶不同参数下的病毒效价情况进行了比较和优化,最终确定培养参数为:以1:10稀释种毒后接种、接种24 h的PK15细胞、5～7 mg/mL微载体浓度和维持液低糖DMEM+2 g/L水解乳蛋白(LH)的培养方式效果最为理想;采用微载体和反应器系统生产PCV2,病毒效价可达10^(5.0)TCID_(50)/mL以上。The production of PCV2 was studied in three cultural systems-bioreactor,roller bottle and square bottle,and the titers of PCV2 under different parameters of roller bottle were compared and optimized.The experiments showed that the optimal culture result could be obtained by inoculating PK15 cells for 24 h with the virus-seed of 1：10 dilution and using microcarrier concentration of 5～7 mg/mL and maintenance medium of low sugar DMEM containing 2 g/L lactoalbumin hydroly-sate. The titer of the virus produced by the cells cultured on minrocarriers in a bioreactor could reach above 10^（5.0） TCID_（50）/mL.

关 键 词：PCV2 微载体培养 滴度 培养条件 

分 类 号：S852.65[农业科学—基础兽医学]