内吗啡肽-1对正常人骨髓基质细胞造血调控的研究  

Effect of Endomorphin-1 on Hematopoietic Regulatory Molecules from Normal Human Bone Marrow Stromal Cells

作　　者：赵丽[1] 李婷[1,2] 周兰霞[1] 

机构地区：[1]兰州大学第一医院中心实验室,甘肃兰州730000 [2]甘肃省兰州市甘肃省妇幼保健院

出　　处：《中国实验血液学杂志》2010年第6期1574-1578,共5页Journal of Experimental Hematology

基　　金：甘肃省科技攻关项目;编号:2GS042-A43-014-19

摘　　要：本研究探讨内吗啡肽-1对正常人骨髓基质细胞表面黏附分子细胞间黏附分子(ICAM-1)和血管细胞黏附分子(VCAM-1),以及基质细胞分泌的细胞因子IL-6、TNF-α的调控作用。应用流式细胞术检测黏附分子ICAM-1和VCAM-1的表达,用ELISA法检测细胞因子IL-6、TNF-α的浓度。结果表明:加入内吗啡肽-1培养24小时后ICAM-1和VCAM-1表达与对照组比较有显著性差异(p<0.05),但内吗啡肽-1浓度的改变对于二者的表达无影响;IL-6、TNF-α的浓度实验各组数据与对照组比较无显著性差异(p>0.05)。结论:内吗啡肽-1能调控正常骨髓基质表面ICAM-1和VCAM-1的表达;对于正常骨髓基质细胞分泌的细胞因子IL-6、TNF-α无促进或抑制作用。The aim of study was to investigate the regulatory effect of endomorphin-1（EM-1） on ICAM-1 and VCAM-1 on normal human bone marrow stromal cell surface as well as IL-6 and TNF-α excreted by human normal bone marrow stromal cells. The expression of ICAM-1 and VCAM-1 was determined by flow cytometry, and the concentration of IL-6 and TNF-α was detected by ELISA. The results indicated that the expression of ICAM-1 and VCAM-1 was significantly different from the control group after being cultured with endomorphin-1 for 24 hours （p0.05）, and different concentrations of endomorphin-1 had no effect on expressions of ICAM-1 and VCAM-1 both; there was no diffe-rence in secretion of IL-6 and TNF-α between experimental and control groups （p0.05）. It is concluded that endo-morphin-1 regulates the ICAM-1 and VCAM-1 expression on human normal bone marrow stromal cell surface, but endomorphin-1 does not display any effect on IL-6 and TNF-α secreted by human normal bone marrow stromal cells.

关 键 词：内吗啡肽-1 骨髓基质细胞 造血调控 

分 类 号：R973[医药卫生—药品] R331.2[医药卫生—药学]