调节性CD4^+T细胞及非调节性CD4^+T细胞与HIV/AIDS患者疾病进展关系的研究  

Relationships between Regulatory CD4^+ T Cells or Non-regulatory CD4^+ T Cells and Disease Progression in HIV/AIDS Patients

作　　者：画伟[1] 焦艳梅[1] 田亚坤[1] 李娟[1] 计云霞[1] 王蕊[1] 张彤[1] 陈德喜[1] 吴昊[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染中心

出　　处：《首都医科大学学报》2010年第6期706-710,共5页Journal of Capital Medical University

基　　金：国家"十一五"传染病重大专项(2008ZX10001-006);北京市科委资助项目(D0906003040391)~~

摘　　要：目的分析比较中国HIV感染者CD4+CD25+Foxp3+调节性T细胞(CD4+regulatory T cells,Treg)及非Treg细胞与疾病进展的关系,探讨Treg细胞在HIV感染过程中的作用。方法选取76例HIV/AIDS患者,根据其CD4+T细胞计数水平不同分为3组,A组CD4<200个/μL,B组CD4200～400个/μL,C组CD4>400个/μL。采用流式细胞仪胞内染色技术检测CD4+CD25+Foxp3+调节性T细胞的水平,并分析其与CD4计数及病毒载量的相关性。结果随着疾病的进展,Treg细胞百分比逐渐升高,各组间差异有统计学意义;Treg细胞及非Treg细胞的绝对计数均明显下降,且以非Treg细胞下降为主;Treg绝对计数与病毒载量呈负相关(P<0.05)。结论中国HIV感染者随着疾病进展,辅助性T细胞等非Treg细胞的数量下降,对机体免疫保护能力降低,而Treg细胞数量及功能的下降使其对机体的过度免疫活化的抑制作用减弱,病毒复制,加剧病情进展。Objective To analyze the relationships between CD4＋CD25＋Foxp3＋ regulatory T cells（Treg） or non-CD4＋CD25＋Foxp3＋ regulatory T cells（non-Treg） and disease progression in Chinese HIV-infected individuals,and thereby to investigate the role of Treg in the process of HIV-1-infection.Methods Seventy-six HIV-infected adult patients were enrolled in this study.According to their CD4＋ T cell counts in peripheral blood,patients were divided into Group A（CD4200/μL）,Group B（CD4 200～400/μL） and Group C（CD4400/μL）,respectively.The CD4＋CD25＋Foxp3＋ regulatory T cells were examined with intracellular staining flow cytometric technique.The relationship between CD4＋ T cells counts and the plasma HIV-1 viral load was analyzed.Results With the progression of disease the percentages of Treg cells were raised gradually,and this reached statistical significance;compared with the healthy control,both the absolute counts of Treg cells and non-Treg cells decreased significantly in HIV-infected individuals and mainly in the non-Treg cells.Furthermore,the absolute numbers of Treg cells were inversely correlated with plasma HIV-1 RNA viral load（r=-0.334,P0.05）.Conclusion With disease progression in Chinese HIV-1-infected patients,the reduction in the frequency of non-Treg cells,such as helper T cells（Th）,dampened the immune protection,while the diminished frequency and function of Treg cells decreased the inhibition of the excessive immune activation,which may favor viral replication and drive HIV disease progression.

关 键 词：调节性T细胞 人类免疫缺陷病毒 人类免疫缺陷病毒载量 

分 类 号：R512.91[医药卫生—内科学]