慢性铝过负荷致神经元退变大鼠海马金属离子含量变化  

Metal ion levels of hippocampal neuron in chronic aluminum overload-induced neurodegenerative rats

作　　者：余丽娟[1] 杨俊卿 苏强[1] 张鹏[1] 

机构地区：[1]重庆医科大学药学院药理教研室重庆市生物化学与分子药理学重点实验室,重庆400016

出　　处：《中国老年学杂志》2010年第23期3500-3502,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(30672211)

摘　　要：目的观察慢性铝过负荷致神经元退变大鼠海马金属离子含量的变化。方法葡萄糖酸铝(A l3+200 mg/kg)灌胃给予Wistar大鼠,1次/d,每周连续5 d,共20 w,建立慢性铝过负荷致神经元退变大鼠模型。Morris水迷宫检测大鼠空间学习记忆能力,HE染色观察大鼠海马神经元形态变化,等离子体原子发射光谱检测大鼠海马金属离子含量。结果慢性铝过负荷致大鼠空间学习记忆能力明显下降(P<0.01),海马神经元核固缩;脑内铝、铁、锰、铜和锌含量明显增加(均P<0.01)。结论慢性铝过负荷致大鼠神经元退变与大鼠海马金属离子含量增加有关。Objective To investigate the variation of metal ion levels（Cu,Zn,Mn,Fe,Al） of chronic aluminum overload-induced neurodegenerative rat hippocampus.Methods The neurodegenerative models of Wistar rats were established via intragastric administration of aluminum gluconate（Al3＋ 200 mg/kg）,once a day, 5 days per week,for 20 weeks.Spatial learning and memory function of rat were evaluated by Morris water maze.Pathomorphologic changes of hippocampal neurons were observed by HE staining.The metal ion levels of rat hippocampus were detected by ICP-AES.Results The spatial learning and memory function of chronic aluminum overload rat were significantly impaired.Hippocampal neurons in model rats showed obvious karyopycnosis.The metal ion levels（Cu,Zn,Mn,Fe,Al） were obviously increased.Conclusions There is a close relationship between neurodegeneration induced by chronic aluminum overload in rats and the increase of metal ion levels in rat hippocampal neuron.

关 键 词：神经元退变 金属离子 铝过负荷 

分 类 号：R995[医药卫生—毒理学]