农杆菌介导的下胚轴法转化大豆研究  

Study on Hypocotyl-based Agrobacterium-mediated Transformation of Soybean

作　　者：罗秋兰[1] 周失[1] 赵琳[1] 李永光[1] 李文滨[1] 

机构地区：[1]东北农业大学教育部大豆生物学重点实验室,黑龙江哈尔滨150030

出　　处：《大豆科学》2010年第6期925-929,共5页Soybean Science

基　　金：国家自然科学基金面上项目(30971810)

摘　　要：以下胚轴为外植体,采用农杆菌介导方法成功将大豆的蓝光受体启动子pGmPLP1导入大豆基因组中,并且该基因在后代中得到稳定遗传表达。比较了栽培品种黑农44用子叶节法和下胚轴法转化的区别,发现用子叶节法不能获得高效转基因植株的品种可以顺利通过下胚轴法进行转化。The study successfully transformed the soybean blue light receptor gene promoter pGmPLP1 into soybean genome by hypocotyl-based Agrobacterium-mediated transformation method,and the gene was steady expressed in progenies.Also the comparison between cultivar Heinong 44 transformed by cotyledon nodes and hypocotyls was conducted.The results showed that the cultivars that cannot efficiently transformed by cotyledon nodes method could successfully transformed by hypocotyl method.

关 键 词：大豆器官发生途径 再生率 丛生芽 下胚轴 

分 类 号：S565.1[农业科学—作物学]