固相酶法分离大豆胰蛋白酶抑制剂研究  

Separating Soybean Trypsin Inhibitors with Solid-Phase Enzyme Method

作　　者：陈滴[1] 刘银燕[1] 杨锦竹[1] 王广树[1] 杨晓虹[1] 

机构地区：[1]吉林大学药学院,吉林长春130021

出　　处：《大豆科学》2010年第6期1056-1058,共3页Soybean Science

摘　　要：以硅胶-壳聚糖为载体,通过戊二醛偶联胰蛋白酶,制备固定化酶,分离纯化大豆中胰蛋白酶抑制剂。对戊二醛浓度、反应温度、酶浓度等影响固定化酶制备的因素进行了研究。戊二醛的浓度为2.0%,反应温度为20℃,胰蛋白酶与载体比为15∶1 000时,分离纯化的大豆胰蛋白酶抑制剂活性最高,达1872.51 U.mg-1,比粗品高46.22倍。结果表明通过该法能够获得较高活性大豆胰蛋白酶抑制剂,为大豆胰蛋白酶抑制剂的开发利用提供了依据。In order to separate and purify the soybean trypsin inhibitors,immobilized trypsin was prepared by coupling siliconechitosan activated carriers with trypsin using glutaraldehyde as coupling agents.The influence factors of coupling reaction including glutaraldehyde concentration,reaction temperature and trypsin concentration were investigated and the results showed that the best conditions were as follow： the concentration of glutaraldehyde was 2.0%,the temperature was 20℃,and the ratio of trypsin with silicone-chitosan was 15∶1 000.The activity of soybean trypsin inhibitors isolated by the above immobilized trypsin was 1872.51 U·mg^-1,and the purified degree increased 46.22 times.The results suggested that soybean trypsin inhibitors with higher activity can be obtained by this method and it is a better way to exploit the soybean trypsin inhibitors.

关 键 词：固相酶法 大豆 胰蛋白酶抑制剂 

分 类 号：TQ464[化学工程—制药化工]