四种活体染色法评定家禽精子形态的研究  

Evaluation of Fowl Sperm Morphology Using Four Staining Methods

作　　者：吴慧英[1] 贾汝敏[1] 

机构地区：[1]广东海洋大学动物科学系,广东湛江524088

出　　处：《中国家禽》2010年第23期15-18,共4页China Poultry

基　　金：广东省教育部产学研结合项目(2010B09040268);广东海洋大学学生创新项目基金

摘　　要：精子形态学检测是预测精子功能最有价值的指标之一,形态异常精子越多受精率越低。用4种伊红-苯胺黑活体染色法(Blom、Bakst、Morisson、Jaskowski)评价三黄鸡和贵妃鸡的精子形态,结果表明:染色方法显著影响精子形态,但不影响精子活率,染液对精子自然形态的保持至关重要。Blom染色法导致精子头部膨胀,畸形精子显著增多(P≤0.01),Bakst、Morisson和Jaskowski染色法均可作为评定家禽精子形态的实用方法,其中Jaskowski染色方法最简便经济,值得在家禽生产中推广应用。Sperm morphology is the most valuable indicator for predicting the fertilising ability of spermatozoa ,and as the proportility of abnormal sperm become higher the fertility get lower. Four kinds of in vivo staining methods（Biota, Bakst,Morisson,Jaskowski）,based on eosin-nigrosin smears,were used to evaluating rooster sperm morphology of Guangxi three-yellow local chickens and Princess chickens. The result showed that staining methods had a significant influence on spermatozoa morphology,while no impact on sperm motility. Stain plays an important role in keeping sperm normal morphology. Blom＇s staining method caused sperm head swelling and showed a significant increase in the proportion of abnormal spermatozoa （P〈0.01）,and those of Bakst,Morisson and Jaskowski were very convenient in determining fowl sperm morphology. And Jaskowski＇ staining method was the most ecnomic and shoud be recomended in evaluating fowl sperm morphology in field.

关 键 词：精子活体染色法 精液 精子形态 鸡 

分 类 号：S83[农业科学—畜牧学]