嫁接技术在大豆花叶病毒长距离运输机制研究上的应用  

The application of graft technique in the soybean for investigation of soybean mosaic virus transporting mechanism

作　　者：姚贵滨[1] 李文龙[1] 赵永山[1] 郑文永[1] 王冬梅[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《河北农业大学学报》2010年第6期74-78,共5页Journal of Hebei Agricultural University

基　　金：国家自然科学基金资助(30971706);河北省自然科学基金资助(C2008000321)

摘　　要：大豆花叶病毒侵染大豆植株后,通过胞间连丝进行胞间运输,通过维管束系统完成长距离运输。传统的摩擦接种只适合研究胞间运输,在研究大豆花叶病毒长距离运输方面,目前还没有一个较好的方法能够将大豆花叶病毒接种到维管束。本试验通过大豆嫁接过程中苗期的选择、切口长度以及切口的处理等条件进行了摸索,利用嫁接技术将病毒接种到维管束,为研究病毒的长距离运输机制提供技术保障。After infected the plants of soybean,soybean mosaic virus（SMV） will be transported between the cell through the plasmodesmata and complete the long-distance transported through the vascular bundle.The traditional mechanical inoculation,friction inoculation,is only applicable to study cell-to-cell movement.Nowadays,in the study area of virus long-distance transport,there is non of a better way to let the SMV inoculate to the vascular.This paper will explore the conditions of the seedling selection,length of incision and the incision of the processing conditions,using grafting techniques,to let the SMV inoculate to the vascular.It provided the ensure of technology for studying of virus long-distance transport.

关 键 词：嫁接 大豆花叶病毒 CP 

分 类 号：S565.1[农业科学—作物学]