增强UV-B辐射对小麦叶肉细胞原生质体微丝骨架的影响  

Influence of enhanced UV-B radiation on microfilament cytoskeleton in wheat mesophyll protoplast

作　　者：翟菁如[1] 陈慧泽[1] 高丽美[1] 李永峰[1] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院,临汾041004

出　　处：《生物学杂志》2010年第6期33-36,共4页Journal of Biology

基　　金：国家自然科学基金资助项目(30671061);山西省自然科学基金资助项目(20081101)

摘　　要：以增强UV-B(10.08 kJ/m2.d)辐射后的小麦幼苗叶肉细胞原生质体为材料,异硫氰酸荧光素标记的鬼笔环肽(FITC-Ph)为探针,利用激光共聚焦扫描显微镜,观察分析小麦叶肉细胞原生质体中微丝骨架的分布及形态变化。结果表明对照组中,叶肉细胞原生质体微丝呈现网状或平行状随机排列,形态上表现为纤维状结构。增强UV-B辐射处理后,原生质体中微丝的密集分布遭到破坏,纤丝状微丝消失,聚集成束,或呈点状、碎片状分布。Microfilaments（MFs） were observed by confocal laser scanning microscopy（CLSM） in the wheat mesophyll protoplast treated with FITC-Phalloidin.Two types of MFs bundles could be recognized.They were beneath the plasma membrane parallel to each other surrounding the chloroplast.When the wheat cells were treated by enhanced ultraviolet-B radiation,the distribution pattern of MFs was changed form the form of net to bundles or granules.

关 键 词：增强UV-B 小麦 原生质体 微丝 激光共聚焦扫描显微镜 

分 类 号：Q245[生物学—细胞生物学]