流体剪切应力对大鼠骨髓间充质干细胞MT1-MMP基因表达的影响  

Effects of laminar shear stress on expression of MT1-MMP in rat bone marrow-derived mesenchymal stem cells

作　　者：唐杰[1] 孙晓东[2] 袁雅红[3] 李瑞明[3] 彭兴春[1] 王汉琴[1,3] 

机构地区：[1]湖北医药学院解剖学教研室 [2]湖北医药学院基础医学研究所 [3]胚胎干细胞研究湖北省重点实验室,湖北医药学院附属太和医院,湖北十堰442000

出　　处：《中国临床解剖学杂志》2010年第6期680-682,共3页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(30770535);湖北省高等学校优秀中青年科技创新团队计划(T201008);湖北医药学院博士启动金(2007QDJ7)

摘　　要：目的探讨流体剪切应力对大鼠骨髓间充质干细胞(ratbonemarrow-derived mesenchy malstem cells,rMSCs)中膜型基质金属蛋白酶1(Membrane type-1 matrix metalloproteinase,MT1-MMP)基因表达的影响,为阐明应力诱导rMSCs分化的分子机制提供一些实验依据。方法应用平行平板流动腔系统,给rMSCs施加不同大小和不同加载时间的层流剪切应力,用real-time PCR检测MT1-MMP基因mRNA的表达水平。结果 5,15,30 dynes/cm2剪切应力均能刺激rMSCs的MT1-MMP mRNA表达,且其作用呈时间依赖和强度依赖。p38抑制剂(SB202190)可以明显抑制这种作用,而PI3K抑制剂(wortmannin)却增强了这种效果。结论流体剪切应力可诱导rMSCs的MT1-MMP基因表达,其表达量与刺激时间和剪切应力的强度密切相关,这种作用可能通过p38MAPK和PI3K/Akt信号通路。Objective To investigate the effect of laminar shear stress on the expression of membrane type-1 matrix metalloproteinase（MT1-MMP）in rat bone marrow-derived mesenchymal stem cells（rMSCs）.Methods rMSCs were isolated from SD rat lower extremities and loaded by laminar shear stress with the parallel-plate flow chamber system,then the expression of MT1-MMP mRNA of rMSCs was examined by real-time PCR.Results Shear stress with different magnitude of 5,15,30 dynes/cm2 all induced MT1-MMP mRNA expression,especially high shear stress,30 dynes/cm^2,which was time-dependent.The expression of MT1-MMP mRNA was inhibited by SB202190,an inhibitor of p38,but enhanced by wortmannin,an inhibitor of PI3K/Akt.Conclusion Shear stress can activate the expression of MT1-MMP.The amount of MT1-MMP expression is closely related to stimulating time and the strengths of shear stress,and p38MAPK and PI3K/Akt signal pathway may play a critical role during the process.

关 键 词：间充质干细胞 膜型基质金属蛋白酶1 剪切应力 

分 类 号：R318.01[医药卫生—生物医学工程]