版纳微型猪近交系胎儿成纤维细胞系的建立及其生物学特征  

Establishment and biological characteristics of fetal fibroblast cell lines in Banna minipig inbred line

作　　者：李红[1] 魏红江[1] 许成盛[1] 汪霞[2] 卿玉波[1] 曾养志[1] 

机构地区：[1]云南农业大学云南省版纳微型猪近交系重点实验室,云南昆明650201 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《湖南农业大学学报（自然科学版）》2010年第6期678-682,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31060308);云南省教育厅项目(07Z11542)

摘　　要：以版纳微型猪近交系妊娠47d的胎儿为材料,采用胰蛋白酶消化法消化培养胎儿成纤维细胞,对其进行细胞形态观察、细胞冻存前和复苏后存活率测定、生长曲线绘制和染色体核型分析等生物学特征检测.结果表明,培养的版纳微型猪近交系胎儿成纤维细胞呈典型的成纤维细胞形态;细胞冻存前和复苏后的存活率分别为98.06%和92.12%;生长曲线呈"S"形,倍增时间为36h;对所制备染色体核型进行分析,显示2n=38,XY,并在体外培养14个代次后仍能保持正常核型.Useing a 47 d fetus of Banna minipig inbred line as materials,fetal fibroblast cells were dispersed in vitro with trypsinnization and then subcultured.Observations on morphology,determination of viability,drawing of dynamic growth,and analysis of karyotype were carried out.The results showed that the cell was morphologically consistent with fibroblasts,cell viability was 98.06% before freezing and 92.12% after thawing,the growth curves was sigmoidal with population doubling time（PDT）of cells was approximately 36 h,karyotyping indicated a total chromosomes number 2n=38,sex chromosomes were XY,and the karyotype is normal afrer cultured up to passage 14 in vitro.

关 键 词：版纳微型猪近交系 胎儿成纤维细胞 细胞培养 生物学特征 

分 类 号：S858.28[农业科学—临床兽医学]