肉鸡鸡白痢沙门菌的分离鉴定  

Isolation and Identification of Salmonella pullorum from Broiler

作　　者：王春丽[1,2] 姜北宇[1] 曹峰子[1,3] 陈小玲[1] 张培君[1] 王宏俊[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100097 [2]吉林农业大学动物科学技术学院,长春吉林130118 [3]河北农业大学动物科技学院,保定河北073000

出　　处：《动物医学进展》2010年第12期153-156,共4页Progress In Veterinary Medicine

基　　金：北京市农林科学院青年基金项目(20090805)

摘　　要：2009年5月,北京郊区某肉鸡养殖户的2 000只7日龄雏鸡出现拉白色稀粪,肝脏有黄色结节等症状和病变的疾病。为探索该病病因及制定有效防治措施,对病鸡样品进行了细菌学检验。通过细菌分离、生化试验、PCR及16 S rRNA基因测序鉴定出1株鸡白痢沙门菌。该分离株感染试验鸡能复制出与其临床表现一致的病例并能引起死亡。表明鸡白痢沙门菌是危害北京郊区该养殖户肉鸡的病原。In May 2009,an enteric outbreak showing blanc-loose stool and flavi-tubercle type of lesion on chick liver occurred among 2000 heads of 7-day-old to 10-day-old chicks in a commercial broiler farm in Beijing suburban area.To identify the pathogen and establish control measures,samples were taken from sick birds and bacteriological examination was performed.Through bacterial isolation,biochemical tests,PCR,16 S rRNA sequencing,a Salmonella pullorum was identified.The chicks infected with the isolate showed similar clinical signs and suffered death as filed birds.The laboratory examination confirmed that the Salmonella pullorum was the etiological agent on the broiler farm in Beijing suburban area.

关 键 词：肉鸡 鸡白痢沙门菌 实验室检验 

分 类 号：S852.612[农业科学—基础兽医学]