银杏CONSTANS基因的克隆与序列分析  

Molecular Cloning and Sequence Analysis of CONSTANS Gene from Ginkgo biloba

作　　者：龚付全[1] 熊超[1] 陈柳吉[1] 宁迎晶[1] 张威威[1] 许锋[1] 程水源[2] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025 [2]黄冈师范学院生命科学与工程学院,湖北黄冈438000

出　　处：《湖北农业科学》2010年第12期2945-2948,共4页Hubei Agricultural Sciences

基　　金：湖北省自然科学基金(2009CDB232);教育部科学技术研究重点项目(210137);湖北省教育厅科学研究计划优秀中青年人才项目(Q20091201);长江大学博士基金(0010113);国家大学生创新性实验计划(101048942)

摘　　要：利用1对简并引物,从银杏中克隆得到CONSTANS基因的cDNA片段,命名为GbCO。GbCO长855bp,编码285个氨基酸。核苷酸和蛋白质序列多重比较结果显示,GbCO与其他植物的CO基因的核苷酸与蛋白质序列均高度同源。GbCO编码的蛋白质与挪威云杉、海岸松和长白松的CO氨基酸相似性分别为60%、53%和52%。CO系统进化树显示,GbCO与其他裸子植物亲缘关系较近。该研究通过克隆GbCO基因,可为利用转基因技术缩短银杏童期提供基因资源,也为进一步了解银杏开花的分子调控机理奠定基础。The cDNA fragment of CONSTANS（CO） gene was cloned from Ginkgo biloba using a pair of degenerated primers; and named as GbCO. GbCO was 855bp long and encoded 285 amino acids; and has high nuclectide and amine-acid homology with CO genes of other plants. It’s proteir homoloy with the CO gere of Picea abies, Pinus pinaster and Pinus sylvestris was 60%,53% and 52% respectively. Phylogenetic tree analysis revealed that GbCO had a closer relationship with COs from Gymnosperm plants than from other plants. The cloning of GbCO could be a basis for the study of G. biloba flowering regulation mechanism, and could provide genetic resources to shorten the phase of G. biloba juvenility by transgenic technology.

关 键 词：银杏 CONSTANS 基因克隆 序列分析 

分 类 号：Q522.6[生物学—生物化学]