仔猪大肠埃希氏菌病、C型产气荚膜梭菌病二联灭活疫苗的效力和安全试验  

Efficacy and Safety of Inactivated Escherichia coli and Clostridium perfringens Type C Combination Vaccine in Piglets

作　　者：邓均华[1] 刘泽文[1] 杨克礼[1] 梁望旺[1] 伍锐[1] 段正赢[1] 周丹娜[1] 郭锐[1] 徐涤平[1] 田永祥[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,武汉430064

出　　处：《湖北农业科学》2010年第12期3133-3136,共4页Hubei Agricultural Sciences

基　　金：湖北省科技厅引导项目(2006AA201C25);湖北省农业科技创新中心资助项目(2007-620-001-03)

摘　　要：将带有K88、K99和987P纤毛抗原的大肠埃希氏菌(Escherichia coli)菌株(C83549、C83644和C83710)和免疫原性良好的C型产气荚膜梭菌(Clostridium perfringens type C)菌株(C59-2、C59-37和C59-38)第10代基础种子制备的原液进行浓缩、甲醛溶液灭活和脱毒后,按抗原含量,加氢氧化铝胶制成3批二联灭活疫苗。经过对免疫怀孕母猪所产仔猪的攻毒测定,得到疫苗最小的免疫剂量为每头2mL,确定使用剂量为每头4mL。疫苗的免疫攻毒试验结果表明,疫苗在怀孕母猪中采用颈部肌肉注射进行免疫,二次免疫才能使仔猪通过吮吸初乳获得80%以上的被动免疫保护能力,与单苗具有相似的免疫保护力;单剂量、单剂量重复和超剂量的安全性试验结果显示,3批二联灭活疫苗不会引起母猪的全身不良反应和明显的局部反应,无流产,胎儿发育完全正常。Three batches of inactivated combination vaccine were prepared by cultivation, concentration, inactivation and detoxification of Escherichia coli with K88, K99 and 987P cilium antigens （C83549, C83644 and C83710） and Clostridium perfringens type C （C59-2, C59-37 and C59-38）. The results of efficacy tests showed that the minimal effective dosage of the inactivated combination vaccine was 2 mL, then the recommended dose was 4 mL. Over 80% passive immune protection in piglets was obtained through sucking colostrums of immunized pregnant sows. Moreover, there was no significant difference between combination vaccine and single vaccine. No side-effect was observed in sows and piglets after injection with single, repetitive and super doses.

关 键 词：大肠埃希氏菌 C型产气荚膜梭菌 二联灭活疫苗 效力试验 

分 类 号：S852.61[农业科学—基础兽医学] S852.42[农业科学—兽医学]