正交试验法优选细梗胡枝子中总黄酮提取工艺  

Optimization of the Extraction Technology of Total Flavoncids from Lespedeza virgata by Orthogonal Test

作　　者：黄志勤[1] 程齐来[1] 李洪亮[1] 

机构地区：[1]赣南医学院药学院,江西赣州341000

出　　处：《湖北农业科学》2010年第12期3168-3169,3178,共3页Hubei Agricultural Sciences

基　　金：江西省自然科学基金项目(2009GQY0015);江西省教育厅科技计划项目(GG09321)

摘　　要：研究了中药细梗胡枝子中总黄酮最佳提取工艺。采用正交试验,根据各因素的影响大小确定细梗胡枝子中总黄酮的最佳提取条件,通过紫外分光光度法测定总黄酮的含量。结果表明,细梗胡枝子中总黄酮的最佳提取工艺为乙醇体积分数40%、料液比1∶8(W/V,g∶mL)、提取时间1h。To optimize the extraction process of total flavonoids in Lespedeza virgata, single factor experiment was conducted. The content of total flavonoids was determined by UV spectrophotometer. The influence of each factor was measured in orthogonal test. The results showed that the optimum conditions of extraction technique were establishied as follows：40%（V/V） ethanol,the ratio of solvent to material 1∶8（W/V,g∶mL） and extracting 1 h each time.

关 键 词：细梗胡枝子 总黄酮 正交试验 提取工艺 

分 类 号：R284.2[医药卫生—中药学]