根瘤菌的RAPD分子标记技术检测和耐盐碱筛选  

Technical detection of RAPD molecule maker and saline alkali tolerance experiments on Rhizobia

作　　者：陈利云[1] 张海林[1] 周志宇[2] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741000 [2]兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《干旱地区农业研究》2010年第6期212-216,共5页Agricultural Research in the Arid Areas

基　　金：国家重点基础研究发展规划项目(973项目)(2007CB108903)

摘　　要：采用17株分离于苜蓿和草木樨的根瘤菌为研究对象,进行了RAPD分子标记技术检测和耐盐碱筛选实验。结果显示:供试根瘤菌菌体基因组RAPD电泳图谱分为6组不同的条带,说明17株菌中有6个不同菌系。不同菌系的根瘤菌耐盐碱筛选试验表明,来自临泽的根瘤菌耐盐碱能力均比来自天水的强,其中两株能在pH14的YMA培养基上生长,一株可耐1.539 mol/L的NaCl,两株能耐1.971 mol/L的Na2SO4,尤其菌GL007对高pH和两种盐都有较强的忍耐性,是实验筛选出的珍贵耐盐碱菌种资源。With 17 Rhizobia strains isolated from nodules of alfalfa and sweet clover as the research objects,RAPD detection and salt and alkali tolerance screening tests were conducted.The results showed that there were 6 different bacterial systems in 17 Rhizobia.The experiment of salt and alkali tolerance showed that rhizobia of alfalfa in Linze had better stress tolerance ability than that in Tianshui.Two strains could grow well under pH 14,one strain could grow at medium containing 9% NaCl and two strains could tolerate 1.971 mol/L Na2SO4 on YMA medium.All these data showed GL007 strain had strong tolerance ability for salt and high pH.It was a precious strain resource of saline-alkali tolerance.

关 键 词：根瘤菌 RAPD技术 筛选试验 耐盐碱 

分 类 号：S154.381[农业科学—土壤学]