鸡传染性囊病二价活疫苗攻击保护滴度的测定  

Detection of Protective Titers of Bivalent Live Infectious Bursal Disease Vaccine Against Virulent IBD Viruses

作　　者：刘爵[1] 刘有昌[1] 姚炜光[1] 周蛟[1] 王平[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所

出　　处：《华北农学报》1999年第2期133-138,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家科委"八五"科技攻关项目

摘　　要：使用ＳＰＦ雏鸡进行鸡传染性囊病二价活疫苗的攻击保护滴度的测定。ＳＰＦ雏鸡１４日龄免疫，于免疫后７ｄ、８ｄ、９ｄ、１０ｄ、１２ｄ、１４ｄ采血进行ＩＢＤ中和抗体的测定，同时分别进行标准Ｉ型强毒ＣＪ８０１株和标准变异株强毒１０８４Ａ株的攻击，建立抗体滴度与攻击保护率之间的直线回归方程。二价苗免疫后１４ｄＩＢＤ中和抗体滴度达到２９６８（ＧＭＴ），其对ＣＪ８０１株和１０８４株的攻击保护率分别为８９２１％和８２５９％，从而表明在免疫抗体达到２９６８时，免疫鸡表现对ＩＢＤ强毒良好的攻击保护效果，并将此抗体滴度暂定为鸡传染性囊病二价活疫苗的中和保护滴度。A detection of protective titers of a bivalent live IBD vaccine was conducted using SPF chicks. The chicks were vaccinated at 14 days of age, and were bled and their blood samples and sera collected for the detection of neutralizing antibodies to IBDV in a virusneutralization (VN) assay and simultaneously challenged with either a standard serotype virulent IBDV strain CJ801 or a virulent variant IBDV strain 1084A at 7, 8, 9, 10, 12, 14 days postvaccination, respectively. A rectilinear regression equation between IBD antibody titers and protective rates was established. When the VN antibody titer (GMT, geometric mean titer for vaccinated chicks with the vaccine) increased by 2968, the vaccinated chicks had 89.21% and 82.59% protections against the challenges of strains CJ801 and 1084A, respectively. Consequently, the IBD antibody titer of 2968 (GMT) was tentatively regarded as a protective neutralizing antibody titer resulted from the vaccine against virulent IBD viruses.

关 键 词：鸡 IBD 法氏囊病 二价活疫苗 疫苗 抗体滴度 

分 类 号：S858.315.3[农业科学—临床兽医学]