日本血吸虫病流行区再感染人群血清IgG_4抗体的特异性免疫识别  

Specific Immunobloting by IgG 4 Antibody of Human Reinfected with Schistosoma japonicum in Endemic Area

作　　者：王荣芝[1,2,3] 吴海玮[1,2,3] 陈淑贞 胡林生[1,2,3] 谢彰武 邱英席 邵莉君 曹建平 张绍基 张兆松[1,2,3] 吴观陵 

机构地区：[1]南京医科大学分子免疫寄生虫学研究室 [2]江西省寄生虫病防治研究所 [3]镇江医学院寄生虫学教研室

出　　处：《南京医科大学学报（自然科学版）》1999年第4期287-290,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金

摘　　要：目的对构建人群再感染易感性的预测系统提供有价值的信息。方法采用Ｗｅｓｔｅｒｎｂｌｏｔ技术，以日本血吸虫病流行区化疗后再感染人群的４３份血清中ＩｇＧ４抗体，对日本血吸虫的可溶性虫卵抗原（ＳＥＡ）和可溶性成虫粗抗原（ＳＷＡＰ）进行免疫识别。结果ＳＥＡ中２００ｋｕ、７０ｋｕ及５５～６０ｋｕ蛋白组分和ＳＷＡＰ的８７ｋｕ蛋白组分被２１份以上血清的ＩｇＧ４抗体识别。结论上述血吸虫蛋白分子可能是人群日本血吸虫病再感染易感状态相关的抗原表位。Objective\ A prediction system to human susceptibility of Schistosoma japonicum reinfection was investigated. Methods\ The sera taken from subjects reinfected with Schistosoma japonicum after chemotherapy and had high levels of specific IgG 4 antibody were used to test soluble egg antigens (SEA) and soluble worm antigen preparations (SWAP) by Western blot. Results\ 200 ku, 70 ku and 55￣60 ku protein components of SEA and 87 ku protein of SWAP can be recognized by most of the studied sera. Conclusion\ These results suggested that these protein components may be the antigen epitopes that correlated with human susceptibility of Schistosoma japonicum reinfection.

关 键 词：日本血吸虫病 免疫识别 IGG 再感染 

分 类 号：R532.21[医药卫生—内科学]