神经肽对大鼠胃粘膜血流量的调节作用  

Effects of Neuropeptides on Gastric Mucosal Blood Flow in Rats

作　　者：许宏俐[1] 顾洛[1] 程光[1] 田苏平[1] 李栋生[1] 

机构地区：[1]南京医科大学生理学教研室

出　　处：《南京医科大学学报（自然科学版）》1999年第4期296-299,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的研究某些神经肽对胃粘膜血流量（ｇａｓｔｒｉｃｍｕｃｏｓａｌｂｌｏｏｄｆｌｏｗ，ＧＭＢＦ）的调节作用，并比较其活性大小。方法采用氢气清除法测定大鼠ＧＭＢＦ，分别观察近胃动脉灌注降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎｇｅｎｅ－ｒｅｌａｔｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ）、促胃液素－１７（ｇａｓｔｒｉｎ－１７，Ｇ１７）、甲硫脑啡肽（ｍｅｔ－ｅｎｋｅｐｈａｌｉｎ，Ｍｅｔ－ｅｎｋ）、铃蟾肽（ｂｏｍｂｅｓｉｎ，Ｂｏｍ）、神经肽Ｙ（ｎｅｕｒｏｐｅｐｔｉｄｅＹ，ＮＰＹ）和生长抑素（ｓｏｍａｔｏｓｔａｔｉｎ，ＳＳ）对ＧＭＢＦ的影响。结果①ＣＧＲＰ、Ｇ１７、Ｂｏｍ和Ｍｅｔ－ｅｎｋ均呈剂量依赖性地增加ＧＭＢＦ（Ｐ＜０．０１），其中ＣＧＲＰ作用最强；②一氧化氮（ｎｉｔｒｉｃｏｘｉｄｅ，ＮＯ）生物合成阻断剂Ｌ－ＮＡＭＥ可完全阻断ＣＧＲＰ、Ｂｏｍ引起的ＧＭＢＦ增加效应，而对Ｇ１７、Ｍｅｔ－ｅｎｋ所引起的效应仅部分阻断；③ＮＰＹ、ＳＳ对ＧＭＢＦ有显著抑制作用。结论神经肽对ＧＭＢＦ有显著的调节作用；ＣＧＲＰ、Ｇ１７、Ｂｏｍ和Ｍｅｔ－ｅｎｋ的ＧＭＢＦ增加效应与ＮＯ有关。Objective\ Effects of a few neuropeptides on GMBF was investigated. Methods\ Hydrogen gas clearance technique was used to measure GMBF. Effects of intra arterial infusion of calcitonin gene related peptide (CGRP), gastrin 17 (G 17 ), Bombesin (Bom), Met enkephalin (Met enk), neuropeptide Y (NPY) and somatostatin (SS) on GMBF were observed. Results\ ① CGRP, G 17 , Bom and Met enk significantly increased GMBF in a dose dependent manner. Among these peptides, CGRP was the most potent. ②the gastric mucosal hyperemic responses induced by CGRP and Bom were inhibited completely by L NAME, whereas those induced by G 17 and Met enk inhibited partially. ③ NPY and SS significantly reduced GMBF. Conclusion\ These results indicated that neuropeptides played important roles in controlling GMBF, and that NO was involved in the hyperemic responses caused by CGRP, G 17 , Bom and Met enk.

关 键 词：胃粘膜血流量 神经肽 一氧化氮 氢气清除率 

分 类 号：R322.44[医药卫生—人体解剖和组织胚胎学]