白蜡虫阔柄跳小蜂体内Wolbachia的wsp基因分子检测及序列分析  

Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia in Metaphycus ericeri Xu et Jiang in Ericerus pela

作　　者：杨璞[1] 陈晓鸣[1] 李萌[1] 文灿[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明650224

出　　处：《林业科学研究》2010年第6期823-827,共5页Forest Research

基　　金：中国林科院资源昆虫研究所中央级公益性科研院所基本科研业务费专项资金(7-015-3);云南省应用基础研究基金(2010ZC235);国家自然科学基金青年科学基金(31000983/C040506)资助

摘　　要：采用Wolbachia的通用引物对白蜡虫优势寄生蜂之一,白蜡虫阔柄跳小蜂(Metaphycus ericeriXu et Jiang)体内Wolbachia的wsp基因进行PCR扩增,以此为模板结合Wolbachia的A大组和B大组的特异性引物进行巢式PCR扩增,结果表明:白蜡虫阔柄跳小蜂被A大组和B大组Wolbachia复合感染,将两种寄生蜂感染的A大组和B大组Wolbachia基因片段以及采用通用引物获得的基因片段分别命名为wMeeriA、wMeeriB和wMeeri,对应的片段长度分别为554、439、599 bp。系统发育分析表明:白蜡虫阔柄跳小蜂感染的Wolbachia分属于A大组以及B大组的Con亚组。所获得的序列已经递交到NCBI,登录号分别为:HQ161162、HQ161163和HQ161164。Universal primers were used to amply wsp gene from Metaphycus ericeri DNA by PCR,and the product was used as templates for the following nested PCR amplification using the specific primers for A and B groups of Wolbachia.The results showed that,M.ericeri was infected by both A and B group of Wolbachia.The sequences were named as wMeeriA,wMeeriB and wMeeri,and the length were 554,439 and 599 bp,respectively.Wolbachia in M.ericeri were classified as A-Wolbachia and Con group of B-Wolbachia by phylogenetic analysis of wsp genes.Sequences obtained from this study have been submitted to GenBank and the accession numbers assigned were HQ161162,HQ161163 and HQ161164.

关 键 词：白蜡虫阔柄跳小蜂 WOLBACHIA WSP 

分 类 号：S899.1[农业科学—特种经济动物饲养]