城市污泥堆肥过程中纤维素降解菌的筛选及鉴定  

Isolation and identification of cellulose decomposing microorganisms from sludge composting process

作　　者：崔丽娟[1] 申雪庆[1] 周东兴[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《东北农业大学学报》2010年第11期66-71,共6页Journal of Northeast Agricultural University

基　　金：黑龙江省教育厅科学技术研究项目(11551056);黑龙江省青年科学基金项目(QC2009C40);黑龙江省博士后基金资助;哈尔滨市科技创新人才研究资金项目(2009RFQXN096)

摘　　要：以哈尔滨市文昌污水处理厂的脱水污泥为试验材料,以稻壳作为调理剂,采用自行设计的卧式旋转式污泥好氧发酵装置对污泥好氧发酵过程中纤维素分解菌的筛选进行研究。从城市污泥堆肥的升温阶段分离得到两株纤维素降解真菌,通过形态特征观察、rDNA-ITS序列分析结果表明,两菌株分别为尖孢镰刀菌(Fusariumoxysporum)和脉孢菌(Neurospora sp.),编号为B、E。对两株真菌的纤维素降解特性的研究表明,在以滤纸为唯一碳源的液体培养基中,第10天滤纸失重率分别达到了53%和39%。Investigation had been made in this study to the Isolate and Identify cellulose decom-posing microorganisms during municipal sewage sludge composting mixed with paddy hull under laboratory conditions using horizontal and rotary composting equipment.The two high efficient cellulose decomposing fungi were isolated from temperature rising period of sludge composting process.Through morphous observation and sequence analysis of rDNA-ITS,the results showed that they were Fusarium oxysporum,Neurospora and labeled as B and E,respectively.The decomposition characteristics of B and E on cellulose showed that the weigh losing test of the filter paper reached 53% and 39% after ten days in the liquid culture medium,in which only filter paper was used the carbon source.

关 键 词：城市污泥堆肥 纤维素降解菌 鉴定 

分 类 号：X703[环境科学与工程—环境工程]