利用天然四倍体泥鳅生产全三倍体泥鳅的初步研究  

Study on All-triploid production by crossing Misgurnus anguillicaudatus tetraploids and diploids

作　　者：李雅娟[1] 庞义猛[1] 于卓[1] 张明昭[1] 钱聪[1] 印杰[2] 

机构地区：[1]大连海洋大学生命科学与技术学院,辽宁大连116023 [2]湖北省水产科学研究所,武汉430071

出　　处：《东北农业大学学报》2010年第11期92-96,共5页Journal of Northeast Agricultural University

基　　金：国家科技部科技支撑计划(2007BAD37B04);辽宁省科学技术计划重大;重点项目(2008203001)

摘　　要：2008～2009年利用从湖北省引进的天然四倍体（雄）泥鳅与当地的二倍体（雌）泥鳅杂交,进行全三倍体泥鳅培育的初步研究。结果表明,杂交组（2n×4n）平均受精率、孵化率、成活率分别为54.69%、80.64%、56.64%;对照组（2n×2n）分别为76.23%、82.97%、79.44%。早期胚胎染色体倍性鉴定结果表明,杂交组后代染色体数为71~75条,视为100%三倍体。6月龄时对其杂交后代进行流式细胞仪检测,均为100%三倍体。研究结果表明,利用湖北省洪湖市四倍体泥鳅与当地的二倍体泥鳅杂交能产生100%全三倍体泥鳅,这种生物杂交的方法操作简单、方法可靠,是生产全三倍体泥鳅的最佳途径。Nature tetraploid male loaches collected from Hubei Province were crossed with diploid female from Dalian,and all-triploid loach seeds were produced.The results showed that,the average fertilizing rate,hatching rate and survival rate in the cross group（2n ×4n） were 54.69%,80.64% and 56.64%,respectively,and those in the control group（2n ×2n） were 76.23%,82.97% and 79.44%,respectively.The chromosome number of the early embryos of the progeny was 71-75,which could be considered as 100% triploidy.The result of DNA-content flow cytometry on the 6 months old progeny was also conducted to confirm the ploidy status,showing 100% triploidy.The result of the present research showed that the cross between nature tetraploid loaches from Honghu,Hubei and nature diploid loaches from Dalian could produce 100% triploid loaches.This cross method was simple to operate,reliable,and the best way to produce all-triploid loaches.

关 键 词：泥鳅 二倍体 四倍体 杂交 全三倍体 

分 类 号：S966.4[农业科学—水产养殖]