蛋白酶体抑制剂PS-341对重症急性胰腺炎小鼠的治疗机制  

Therapeutic Mechanism of PS-341 for Severe Acute Pancreatitis

作　　者：胡雅国[1,2] 詹银楚[2] 陈丽萍[3] 姜仁鸦[2] 吴善水[2] 余新春[2] 陈芳建[2] 

机构地区：[1]温州医学院,在职研究生温州325000 [2]衢州市人民医院,衢州324000 [3]衢州职业技术学院,衢州324000

出　　处：《中国中西医结合外科杂志》2010年第6期667-669,共3页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：浙江省科技厅科技计划项目(2006C33077)

摘　　要：目的:探讨蛋白酶体抑制剂PS-341对重症急性胰腺炎的治疗机制。方法:将144只小鼠用完全随机方法分成PS-341治疗组64只、模型组40只和对照组40只。PS-341治疗组在造模前0.5h腹腔注射PS-341(0.5mg/kg)0.2mL;模型组造模前0.5h腹腔注射50%DMSO0.2mL(生理盐水稀释);对照组腹腔注射生理盐水0.2mL。造模后8h将小鼠处死,用自动生化仪检测血清淀粉酶、CRP和LDH,ELISA法检测IL-1β和IL-6。结果:与模型组相比,PS-341治疗组的血淀粉酶、乳酸脱氢酶、C反应蛋白均明显降低(P<0.05),PS-341治疗组血清中IL-1β和IL-6的表达水平较模型组下降(P<0.05,P<0.01),治疗组小鼠胰腺病理组织的炎细胞浸润及出血明显减少(P<0.05)。结论:蛋白酶体抑制剂PS-341可降低SAP小鼠血清淀粉酶、CRP、LDH和IL-1β/6水平和炎细胞浸润及出血,对SAP有一定的治疗作用。Objective To evaluate the therapeutic mechanisms of PS-341 for severe acute pancreatitis in rats. Methods One hundred and forty-four female ICR mice were divided randomly. Sixty-four mice were used in the proteasome specific inhibitor PS-341 treatment group, forty mice in the control group of severe acute pancreatitis without PS-341 treatment and forty were used in the normal group. Severe acute pancreatitis was induced by seven intraperitoneal injections of 50 μg/kg cerulean at hourly intervals; lipopolysaccharide at a dose of 10 mg/kg was administered intraperitoneally 8 hours after the first injection of cerulean. Detections were made on serum amylase, the CRP level and the LDH level by standard clinical methods for automated analysis and IL-1β/6 with ELISA. Results The amylase, CRP level, LDH level and expression of IL-1β/6 in serum decreased in PS-341 treated group as compared with dimethyl sulfoxide （DMSO） treated group （P 0.05, P 0.01）. Conclusion PS-341 inhibited the increase in serum amylase, CRP and LDH, inhibited the increase in serum IL-1β/6. PS-341 might ameliorate SAP in this mouse model.

关 键 词：重症急性胰腺炎 白介素-1Β 白介素-6 PS-341 

分 类 号：R657.51[医药卫生—外科学] Q95-33[医药卫生—临床医学]