PAF拮抗剂红花黄色素对急性心肌梗死大鼠心功能及心肌IL-1β mRNA表达的影响  

Effects of Safflor yellow on heart function and IL-1β mRNA expression of cardiac muscle after acute myocardial infarction in rats

作　　者：李波[1] 邓昌明[1] 吴艳[1] 周大燕[1] 刘地川[1] 黄晶[1] 

机构地区：[1]重庆医科大学附属第二医院心内科,重庆400010

出　　处：《重庆医科大学学报》2010年第11期1668-1671,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨血小板活化因子(Platelet activating factor,PAF)拮抗剂红花黄色素对急性心肌梗死大鼠心功能及心肌白介素细胞-1β(Interleukin-1β,IL-1β)表达的影响。方法:将54只SD大鼠随机分成假手术组、心梗组和红花黄色素治疗组,采用结扎左冠状动脉前降支方法建立急性心肌梗死动物模型,术后24 h治疗组给予腹腔内注射红花黄色素40 mg(/kg.d),心梗组和假手术组给予相同体积的生理盐水腹腔内注射。28 d后用Vivid 7型多普勒超声仪检测各组大鼠心功能,计算左室重量指数,用RT-PCR法检测IL-1βmRNA水平表达。结果:与假手术组相比,心梗组和治疗组心功能明显降低(P<0.05);与心梗组比较,治疗组心功能明显好转(P<0.05)。与假手术组相比,心梗组和治疗组左室重量指数、IL-1βmRNA水平的表达明显增加(P<0.05);和心梗组比较,治疗组左室重量指数及IL-1βmRNA表达减少(P<0.05)。结论:PAF拮抗剂红花黄色素可通过改善急性心肌梗死大鼠心室重构,调节炎性细胞因子IL-1β的表达,从而改善急性心肌梗死大鼠心功能。Objective：To investigate the effects of platelet activating factor（PAF） antagonist Safflor yellow on the heart function and IL-1β mRNA expression of cardiac muscle in rats after acute myocardial infarction.Methods：54 SD rats were randomly divided into sham group,MI group and treatment group;acute myocardial infarction animal models were established by ligation of LAD;rats of treatment group were intervened by injecting intraperitoneally with 40 mg/（kg·d） safflor yellow after 24h;the MI group and sham group were injected with equal volume of saline.The heart functions were detected by Vivid 7-type Doppler ultrasound after 28 days;the left ventricular weight index were calculated and the IL-1β mRNA expression of cardiac muscle was detected by RT-PCR.Results：Compared with sham group,the heart function of MI group and treatment group were significantly depressed （P0.05）;and that of treatment group was better than that of MI group（P0.05）.Compared with sham group,the left ventricular weight index and IL-1β mRNA expression of cardiac muscle in MI group and treatment group were increased significantly（P0.05）;and the left ventricular weight index in treatment group was less than that in MI group;the IL-1β mRNA in treatment group was expressed less than that in MI group（P0.05）.Conclusions： Platelet activating factor（PAF） antagonist Safflor yellow can improve the heart function in rats after acute myocardial infarction by improving left ventricular remodeling and regulating IL-1β expression.

关 键 词：心肌梗死 血小板活化因子(PAF)拮抗剂 心功能 IL-1Β 大鼠 

分 类 号：R541.4[医药卫生—心血管疾病]