一种与不育有关的人精子蛋白质的组织特异性及表达  

Tissue Specificity and Expression of a Human Sperm Protein′s Gene BSD 2 4

作　　者：张梅林[1,2] 周雪峰[1,2] 曹登峰[1,2] 缪时英[1,2] 吴燕婉[1,2] 邢志军[1,2] 宗书东[1,2] 王琳芳[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所医学分子生物学国家重点实验室 [2]国家计划生育委员会科学技术研究所

出　　处：《中国医学科学院学报》1999年第3期166-169,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金;卫生部科学研究基金;863高技术基金

摘　　要：目的通过对已被ＧｅｎＢａｎｋ接受、编码与不育有关的人精子蛋白质的ｃＤＮＡ片段（ＢＳＤ－２．４）的组织特异性及表达阶段的研究，进一步了解其特性。方法采用Ｄｏｔｂｌｏｔ、Ｎｏｒｔｈｅｒｎｂｌｏｔ和组织原位杂交技术。结果该ｃＤＮＡ只在人和大鼠睾丸组织中表达；在人睾丸曲细精管的精原细胞、精母细胞和精子细胞中均有转录。结论ＢＳＤ－２．４ｃＤＮＡ编码蛋白具有睾丸组织特异性，并在精子发生的各个阶段均有不同程度的表达。Objective To investigate the tissue specificity and expression in spermatogenic stage of BSD 2 4 cDNA which might be related with fertility and its sequence was received by GenBank. Methods Dot blot, Northern blot, and in situ hybridization were used. Results Northern blot analysis of sixteen human tissues showed that BSD 2 4 cDNA had one transcript only with testis. BSD 2 4 mRNA was detected in germ cells of human testis. Conclusions BSD 2 4 is a testis specific protein. The cDNA was expressed throughout the stages of the spermatogenic cycle.

关 键 词：人精子蛋白质 组织特异性 组织原位杂交 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]