嗅刺激诱导的c-fos原癌基因在大鼠脑内的表达  

Expression of c fos Prooncogene in Rat Brain Induced by an Olfactory Stimulus

作　　者：杨天祝[1,2] 葛莉[1,2] 万选才[1,2] 

机构地区：[1]河北医科大学 [2]中国医学科学院中国协和医科大学基础医学研究所

出　　处：《中国医学科学院学报》1999年第3期214-220,共7页Acta Academiae Medicinae Sinicae

摘　　要：目的为了解嗅刺激引起中枢神经系统兴奋的区域及程度。方法以醋酸戊酯多次刺激大鼠，１ｈ后灌注固定处死动物。以ｃ－ｆｏｓ探针的ＡＢＣ免疫细胞化学法，观察分析ｃ－Ｆｏｓ蛋白在大鼠脑内标记区。结果中枢系统内ｃ－Ｆｏｓ蛋白标记区十分广泛，尤以脑桥以上脑区为多。除嗅觉通路诸结构外，边缘系统内也有广泛标记，提示存在嗅情绪反应、嗅内脏与嗅躯体反应及嗅记忆等多神经回路的活动。其次，与注意及中枢功能状态调节有关的脑区也有广泛标记。上丘深层、下丘及脑桥核等为强标记，推测与感觉和运动的整合活动有关。结论用嗅刺激诱导ｃ－ｆｏｓ原癌基因表达，确可证实一种特异性嗅刺激可引起大鼠中枢神经系统内泛脑网络上的广泛运动。Objective In order to investigate how widespread the activated regions induced by an olfactory stimulus throughout the central nervous system (CNS), c fos expressions in CNS of the rat observed, which is known as a combined morphologically and functionally probe. Methods Rats were stimulated by iso amyl acetate for about 10 times, then one hour later sacrificed with perfusion of fixatives. After ABC immunocytochemi cal staining with anti c fos polyclonal antiserum, the c Fos protein labelling areas on sections of the brain were observed.Results The results showed that the labelling areas were estremely widespread throughout the brain and cervical segments of the spinal cord in the rat, expecially much more in the areas above the level of the pons. Nearly all neuronal structures in the limbic system were labeled in addition to structures of the olfactory pathways, suggesting there were neuronal activities in multiple neural circuits, which may be associated with emotional, somatic and visceral responses to the olfactory stimulus. Next, many brain areas which were associated with mechanisms of attention and central state regulation were widely labeled. Unexpected heavy labellings in deep layers of the superior colliculus, inferior colliculus and pontine nuclei were presumably relevant to integrating activity between sensations and motions. Conclusions The present study indicated that a specific olfactory stimulus did induce widespread activities of so called pan brain networks within the central nervous system.

关 键 词：嗅觉 C-FOS 原癌基因 免疫细胞化学 

分 类 号：R338.3[医药卫生—人体生理学] R399.12[医药卫生—基础医学]