混合脐血浆在人脐静脉内皮细胞培养中的应用  

Effect of mixed umbilical cord blood plasma on the culture of human umbilical vein endothelial cells

作　　者：李智萍[1] 李金兰[1] 付家瑜[1] 陈珊珊[1] 

机构地区：[1]北京医科大学人民医院血液病研究所

出　　处：《北京医科大学学报》1999年第3期280-282,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：观察混合脐血浆在人脐静脉内皮细胞（ｈｕｍａｎｕｍｂｉｌｉｃａｌｖｅｉｎｅｎｄｏｔｈｅｌｉａｌｃｅｌｓ，ＨＵＶＥＣ）培养中的作用。方法：在含２０％（体积分数）混合脐血浆的内皮细胞培养体系中进行ＨＵＶＥＣ的原代和传代培养，并对培养细胞进行纯度鉴定。同时用噻唑蓝试验观察不同体积分数的混合脐血浆对ＨＵＶＥＣ增殖活力的影响。结果：２０％（体积分数）的混合脐血浆对ＨＵＶＥＣ培养有明显支持作用，该体系培养的ＨＵＶＥＣ经第Ⅷ因子相关抗原（Ⅷ：Ａｇ）和Ⅰ型荆豆凝集素（ＵＥＡⅠ）鉴定纯度可达９３．１％。结论：２０份混合的脐血浆可以代替内皮细胞生长因子成功地用于ＨＵＶＥＣ的培养，以获得高纯度可传代的内皮细胞。Objective: To observe the effect of mixed umbilical cord blood plasma on the culture of human umbilical vein endothelia cells (HUVEC). Methods: Primary and passaged HUVEC were cultured in the medium with 20% mixed umbilical cord blood plasma and their purities were evaluated by Ⅷ:Ag and UEA Ⅰ.Proliferative activities of HUVEC were compared in the medium containing different amounts of mixed umbilical cord blood plasma by methyl thiazolyl tetrazolium(MTT) assay. Results: 20% mixed umbilical cord blood plasma can maintain cell viability in the medium without endothelial cell growth factor(ECGF). The purity of endothelial cells was 93.1%. Conclusion: 20 portion mixed umbilical cord blood plasma can be a substitute for ECGF and used successfully in the culture of HUVEC to obtain high purity and passagable endothelial cells.

关 键 词：混合脐血浆 脐静脉 内皮细胞 培养 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学]