正交实验法优化黄连中小檗碱盐析提取工艺  

Optimization of Salting-out Extraction of Berberine from Huanglian by Orthogonal Experiment

作　　者：王道武[1] 庞雪[1] 肖文[1] 王超[1] 张龙[1] 

机构地区：[1]长春工业大学化学工程学院,吉林长春130012

出　　处：《化学与生物工程》2010年第12期45-48,共4页Chemistry & Bioengineering

摘　　要：采用正交实验法对黄连中小檗碱盐析提取的浸泡时间、黄连粒度、硫酸浓度、HCl调pH值、石灰乳调pH值及NaCl用量等影响因素进行了优化。确定最优的提取方案为:浸泡时间24 h、黄连粒度0.355～0.5 mm、硫酸浓度0.40%,固液比1∶16、石灰乳调pH值12、HCl调pH值5、NaCl用量25%,在此条件下,小檗碱产率为8.4%、纯度为72.7%。各因素对黄连小檗碱提取效果影响大小依次为:固液比>硫酸浓度>浸泡时间>黄连粒度>NaCl用量>石灰乳调pH值>HCl调pH值。Optimization of salting-out extraction of berberine from Huanglian by orthogonal experiment was carried out.The effects of immersion time,Huanglian particle size,the concentration of sulfuric acid,solid-liquid ratio,the pH value adjusted by lime milk,the pH value adjusted by HCl,NaCl amount on the extraction were investigated.The optimum extraction conditions were as follows： immersion time 24 h,Huanglian particle size 0.355～0.5 mm,the concentration of sulfuric acid 0.40%,solid-liquid ratio 1∶16,the pH value adjusted by lime milk 12,the pH value adjusted by HCl 5,NaCl amount 25%.Under above conditions,berberine with yield of 8.4% and purity of 72.7% was obtained.The effect of various factors on berberine extraction was in the order of solid-liquid ratiothe concentration of sulfuric acid 〉immersion time〉Huanglian particle size〉NaCl amount〉the pH value adjusted by lime milk〉the pH value adjusted by HCl.

关 键 词：黄连 小檗碱 提取 盐析 正交实验 

分 类 号：TQ028[化学工程] R284.2[医药卫生—中药学]