山苍子芽器官组培技术研究  

Tissue culture of the Bud Organ from Litsea cubeba

作　　者：蔡玲[1] 王以红[1] 吴幼媚[1] 覃子海[1] 陈博雯[1] 陈贻锋 

机构地区：[1]广西林业科学研究院,广西南宁530002 [2]贺州市八步区林业局,广西贺州542800

出　　处：《西部林业科学》2010年第4期44-47,共4页Journal of West China Forestry Science

基　　金：广西区林业局科技专项"主要木本香料树种优良无性系产业化育苗技术研究"

摘　　要：以山苍子成年树1年生茎段芽为外植体,进行了组培快繁技术实验。结果表明,筛选适宜芽培养的基本培养基以改良MS〔MS＋Ca（NO3）2380 mg.L-1＋H3BO38.2 mg.L-1〕为佳,增殖培养基以改良MS＋BA1.5 mg.L-1＋IBA0.2 mg.L-1＋VC10 mg.L-1＋蔗糖10 000 mg.L-1为佳。在2 000-4 000 lux自然散射光照条件下,山苍子的GLLC-4号、GLLC-3号、GLLC-1号芽继代培养28天,增殖系数分别为5.6、3.1、4.8。We used one year old buds on stem of adult Litsea cubeba as explant tissue to make rapid propagation of the seedlings. The basic medium for Litsea cubeba tissue culture was recommended as an improved Ms [ MS ＋ Ca（NO3）2 380 mg. L^-1 ＋ H3BO3 8. 2 mg L ^-1], the proliferating culture was an improved Ms ＋BA 1.5 mg · L^-1 ＋ IBAO. 2 mg · L^-1 ＋VC 10 mg · L^-1＋ sucrose 10000 mg · L^-1 On the condition of 2 000 - 4 000 lux natural scatted light, the period of sub-culture of GLLC - 4, GLLC - 3 and GLLC - 1 secondary buds was 28 d, and the muhiplication coefficient were 5.6, 3.1 and 4. 8 respectively.

关 键 词：山苍子 芽器官 组织培养 

分 类 号：S718.3[农业科学—林学]