正交试验法优化长春花愈伤组织继代培养条件  

Optimization on Subculture Conditions of Catharanthus Roseus Callus by Orthogonal Test

作　　者：李祥会[1] 伍春莲[1] 房守敏[1] 孙警辉[1] 张思楠[1] 耿文峰[1] 杨军[1] 

机构地区：[1]西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2010年第4期347-351,共5页Journal of China West Normal University(Natural Sciences)

基　　金：四川省教育厅青年基金项目(07ZB089);西华师范大学科研启动基金资助项目(06B026)

摘　　要：研究了不同种类、不同浓度的植物生长调节物质组合对长春花愈伤组织继代培养细胞生长的影响.采用正交实验法设计长春花愈伤组织继代培养基,并连续继代四次.结果发现:经过四次继代后愈伤组织生长旺盛,呈颗粒状,疏松且含水量大,无褐化现象,且最佳继代培养基为MS+6-BA 0.5mg/L+2,4-D 0.5mg/L+Vc 20mg/L.因此通过利用正交试验筛选激素浓度的配比可以对长春花愈伤组织的继代培养条件进行优化,为长春花悬浮细胞培养体系的建立提供最佳材料.The effects of different types,different concentrations of plant growth regulator combinations on callus subculture cell growth were studied in this paper.The subculture mediums of Catharanthus roseus callus were designed by orthogonal test,and callus was subcultured quartic,respectively.The results showed that callus grew rapidly and loosely,took on graininess with maximum water,and was no browning.The best medium for subculture was MS＋6-BA 0.5mg/L ＋2,4-D 0.5ml/L ＋ Vc 20mg/L.Hence,screening different levels of hormones by orthogonal test could optimize the conditions of subculture mediums to gain best materials for suspension cell culture.

关 键 词：长春花 愈伤组织 6-BA 2 4-D 继代培养 

分 类 号：Q945[生物学—植物学]