高效液相色谱法测定六味地黄胶囊中芍药苷含量的实验研究  

Experimental study of determination of paeoniflorin in Liuwei Dihuang capsule by high performance liquid chromatography

作　　者：潘桂玲[1] 韩强[1] 

机构地区：[1]徐州医学院附属医院药剂科,江苏省徐州市221002

出　　处：《临床合理用药杂志》2010年第24期1-2,共2页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的建立六味地黄胶囊中芍药苷含量的高效液相色谱法(HPLC)测定方法。方法采用Kromasil色谱柱,芍药苷以乙腈—水(含0.08%磷酸、0.08%三乙胺)为流动相,检测波长为230nm。结果芍药苷的线性范围为30.52～305.2μg/ml(r=0.9999),平均回收率为100.76%,相对标准偏差(RSD)为1.14%(n=9)。结论 HPLC操作简便、准确、重现性好,能有效控制六味地黄胶囊中芍药苷的质量。Objective To establish a measuring method of high performance liquid chromatography（HPLC） to determine paeoniflorin in Liuwei Dihuang capsule.Methods Kromasil column was adopted,and acetonitrile-water（containing 0.08% phosphoric acid and 0. 08%triethylamine） was taken as the mobile phase. The detection wavelength was 230nm.Results The linear range of paeoniflorin was 30.52 ~305.2μg/ml（r=0.9999）,while the average recovery rate was 100.76%,and RSD was 1.14%（n=9）.Conclusion HPLC has the following advantages：simple,accurate and reproducible,which can control the quality of paeoniflorin in Liuwei Dihuang capsule effectively.

关 键 词：六味地黄胶囊 芍药苷 高效液相色谱法 

分 类 号：R286[医药卫生—中药学]