利用聚乙烯吡咯烷酮防止甘蔗组织培养接种物褐变的研究  

Studies on Preventing Brown Turning of Explant with PVP in Sugarcane (Saccharum officinarum) Tissue Culture

作　　者：陈彪[1] 陈伟栋[2] 梁钾贤 王润华 

机构地区：[1]湛江海洋大学生物技术中心,广东湛江524088 [2]华南农业大学农学系

出　　处：《华南农业大学学报》1999年第3期63-66,共4页Journal of South China Agricultural University

摘　　要：用聚乙烯吡咯烷酮（PVP）预处理甘蔗顶端外植体，并以PVP作为附加成分直接加入培养基，均可取得防止接种物褐变坏死的良好效果；但同时发现，当加入培养基中的PVP达到一定浓度，则会表现出延缓分化芽增殖速度的不良作用；对PVP在植物组织培养中的应用作出评价．When the PVP(Polyvinylpyrrolidone) was used in pretreatment of sugareane explant and usedas an additive in medium, a positive result was obtained which prevented brown turning and necrosis ofinoculum. Meanwhile, it was found that the proliferous rate of differentiated bud was delayed when thePVP concentration in the medium increased in a certain extent. The application of PVP in the plant tissueculture was evaluated affirmatively.

关 键 词：甘蔗 组织培养 接种物褐变 聚乙烯吡咯烷酮 

分 类 号：S566.103.5[农业科学—作物学]