天门冬属4个物种的叶绿体trn L-F序列分析  

TrnL-F Sequence Analysis of 4 Species of Asparagus

作　　者：黄园[1] 王俞人[1] 唐瑶[1] 刘佳蒙[1] 李斌[1] 欧立军[1,2,3] 

机构地区：[1]怀化学院生命科学系,湖南怀化418008 [2]怀化学院湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008 [3]怀化学院民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008

出　　处：《怀化学院学报》2010年第11期62-64,共3页Journal of Huaihua University

基　　金：湖南省高校创新平台开放基金项目(09K106);湖南省科技计划重点项目(2009FJ2008)

摘　　要：对天门冬属4个物种的叶绿体trnL-F序列进行分析发现,trnL-F序列长度约为970bp,GC含量约为33.5%,存在缺失、碱基转化和碱基颠换等形式的变异;基于该序列的聚类系统发育树分为二支,其中天门冬和新疆天门冬为一支,文竹和武竹为另一支.结果认为,trnL-F序列可鉴定不同天门冬属物种,不同天门冬居群的亲缘聚类主要与纬度相关,与经度关系不大.We studied trnL-F sequences variation and affinity of Asparagus.The length of trnL-F sequences were about 970 bp and the percentage of G＋ C contents were about 33.5%.They existed deletion,transformation and transversion.The phylogenetic tree constructed on the basis of the trnL-F sequences showed that 4 species were divided into two branches：Asparagus cochinchinensis（Lour.）Merr.and Asparagus neglectus Kar.et Kir.were a branch and the others were a branch.The results showed trnL-F sequences could identify different Asparagus species.Genetic clustering of different Asparagus populations related primarily to latitude and had the little relationship with longitude.

关 键 词：天门冬属 TRNL-F序列 分子鉴定 

分 类 号：S682.29[农业科学—观赏园艺]