生防菌FJAT-346-PA的内生定殖特性及对香蕉枯萎病的防治效果  

Endophytic colonization of biocontrol bacterium FJAT-346-PA and its efficiency against banana Fusarium wilt

作　　者：余超[1,2] 肖荣凤[1] 刘波[1] 林乃铨[2] 陈璐[1] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福州350003 [2]福建农林大学生物防治研究所,福州350002

出　　处：《植物保护学报》2010年第6期493-498,共6页Journal of Plant Protection

基　　金：公益性行业(农业)科研专项(200903049)

摘　　要：内生生防菌FJAT-346-PA是一株从香蕉植株体内分离的对香蕉枯萎病病原菌具有良好抑制作用的铜绿假单胞菌。为研究该生防菌在植株体内的定殖动态,采用逐步提高诱导浓度的方法,筛选获得稳定的双抗(抗利福平和卡那霉素,浓度均为300μg/mL)菌株FJAT-346-PA-K,跟踪分析了该菌株在香蕉组培苗、盆栽苗及大田植株体内的定殖情况,并调查该菌株对香蕉生长特性的影响和对香蕉枯萎病的防治效果。结果显示:香蕉植株根部和茎部均有该菌定殖,在盆栽苗和大田苗根部的最大定殖量分别为2.15×105CFU/g和8.00×103CFU/g;该生防菌对香蕉有明显的促长作用,显著提高香蕉的株高和叶片数量;对香蕉枯萎病具有较好的防治效果,盆栽防效和田间防效分别为83.67%和82.00%。An endophytic biocontrol bacterium FJAT-346-PA,which was isolated from banana and identified as Pseudomonas aeruginosa,showed high inhibition on mycelial growth of Fusarium oxysporum f.sp.cubense. In order to study the colonization characteristic of FJAT-346-PA inside the plant,an antibiotic-marked experiment was conducted to screen stable antibiotic-marked strain by enhancing the inducing concentration of antibiotic gradually. A double antibiotic-marked strain FJAT-346-PA-K was obtained while the inducing concentration of antibiotic rifampicin and kanamycin were both at 300 μg/mL. After that,the colonization of antibiotic-marked strain FJAT-346-PA-K inside the tissue-culturing seeding,pot-culturing seeding and field plant of banana were analyzed. Meanwhile,the effect of the biocontrol agent on the plant growth and its control efficiency against banana Fusarium wilt banana were also investigated. The results showed that FJAT-346-PA-K could colonize in the roots and stems of banana,with maximum of 2.15×105 CFU/g and 8.00×103 CFU/g at roots of pot-culturing seeding and filed plant,respectively. FJAT-346-PA-K was also found to promote the plant growth remarkably by increasing the plant height and the leaf number. The endophytic biocontrol bacterium performed good control potential against banana Fusarium wilt disease with control efficiencies of 83.67% in the semi-field experiment and 82.00% in the field experiment.

关 键 词：香蕉枯萎病 铜绿假单胞菌 定殖特性 防治效果 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]