AOTF-近红外光谱技术测定前列欣胶囊中欧前胡素、水分、细菌含量的即时快速检测分析  

Real-time Rapid Analysis Method for the Determination of Peulustrin,Moisture Content and Bacterium in Qianliexin Capsule by AOTF-NIR

作　　者：武卫红 董海平[2] 周爱敏[3] 王宁 

机构地区：[1]山东医学高等专科学校,济南市250002 [2]济南金宏利实业有限公司,济南市250100 [3]济南宏济堂制药有限责任公司,济南市250100

出　　处：《中国药房》2010年第48期4590-4594,共5页China Pharmacy

基　　金：山东省自然科学基金资助项目(Y2007C144)

摘　　要：目的:建立测定前列欣胶囊中欧前胡素、水分、细菌含量的即时快速检测分析方法。方法:采用声光可调滤光器-近红外(AOTF-NIR)光谱技术。检测器为InGaAs,测样方式为漫反射,光谱范围为1100～2200nm,波长增量为2.0nm,平均次数为300次。采用偏最小二乘法分别建立校正集样本中5个检测指标的NIR定量分析模型,并对验证集样本进行预测。采用TheUnscram-bler软件分析。结果:欧前胡素和水分含量模型的预测结果较好,NIR预测值与化学值之间的相关系数r分别为0.9993和0.9950,平均RSD为2.44%和1.07%;所建细菌梯度模型则可对不同范围内的细菌个数进行较准确预测,预测结果的平均RSD为6.61%和4.59%。结论:AOTF-NIR为前列欣胶囊的全面质量快速分析提供了一种新方法,而且为中成药固体制剂中水分的快速测定提供了借鉴,为中成药生产过程中细菌等微生物的在线检测做了有益的探索。OBJECTIVE：To establish method for real-time rapidly analysis of peulustrin,moisture content,bacterium of Qianliexin capsules.METHODS：acousto-optic tunable filter-near infrared（AOTF-NIR） spectroscopy.The determination condition was as follows：InGaAs detector,diffusion reflection as determination method,spectrum ranged from 1 100 nm to 2 200 nm,wavelength increment of 2.0 nm,average times of 300.The NIR quantitative analysis models of 5 detection indexes in validation set was established using partial least squares（PLS） method.The using Unscrambler software was adopted to analyze data.RESULTS：The quantitative analysis models of peulustrin and moisture content performed well for prediction,with the correlation coefficients r equal to 0.999 3 and 0.995 0.The mean RSD for the test sets were 2.44% and 1.07%;The gradient model of bacterium can accurately predicted the number of bacteria.The mean RSD for the validation set were 6.61% and 4.59%.CONCLUSION：The method not only provides a new approach for rapid analysis of Qianliexin capsule,but also establishes a foundation for the rapid determination of moisture content in Chinese patent solid preparations and on-line detection of bacteria in the production of Chinese patent medicine.

关 键 词：前列欣胶囊 AOTF-近红外光谱技术 欧前胡素 水分 细菌 即时快速分析 

分 类 号：R917[医药卫生—药物分析学]