8种典型选矿药剂对小鼠的急性毒性研究  

Study on acute toxicity of eight kinds of typical floatation reagent in mice

作　　者：梅光军[1] 高昕[1] 许迪[2] 叶明霞[2] 曹劲松[2] 

机构地区：[1]武汉理工大学资源与环境工程学院,武汉430070 [2]华中科技大学同济医学院实验动物学部,武汉430030

出　　处：《安全与环境学报》2010年第6期1-5,共5页Journal of Safety and Environment

基　　金：国家高技术研究发展计划(863计划)项目(2007AA062123)

摘　　要：为了解8种典型选矿药剂的毒性,选用小鼠灌胃给药做急性毒性试验。在预试验中确定死亡率为0和100%的剂量后,按改良寇氏法计算其中6种药剂LD_(50),并做出比较。最大耐受量法测定其中2种药剂急性毒性作用。正戊基黄药等6种药剂的小鼠灌胃给药范围为101.7～1611 mg/kg时LD_(50)由大到小的药剂依次为丁胺黑药、十二胺、正戊基黄药、水杨羟肟酸、H_(205)和Z_(200);醚胺GE-601、氧化石蜡皂的最大耐受量分别大于5 000 mg/kg、2 703 mg/kg。根据急性毒性分级标准,醚胺GE-601属于实际无毒的物质,正戊基黄药、丁胺黑药、十二胺、氧化石蜡皂、水杨羟肟酸属于低毒物质,而H_(205)和Z_(200)则属于中等毒物质。The present paper is aimed to report the results of our experimental research of the acute toxicity of eight kinds of typical floatation reagents in mice in hoping to know exactly the toxicity degree of the said floatation reagent to humans as well as to other mammals. What we did in our experiments is first of all to choose intragastric control in mice to do acute toxicity test by getting the LD50 or Maximum tolerable dose of the related reagent, so as to be able to provide valuable guidelines for the environmental assessment information. And, then, different methods were applied to perform the tests in hoping to gain preliminary knowledge of the toxic degree of dosage to be used. Next, modified Karber were used to determinate the accurate toxicity to be used in accurate data. Here in our tests, we tried the maximum tolerable dose method to determinate the toxicity up-limit of the reagents in a range from no toxicity in common sense, to very low toxicity and then to the high and finally to the maximum tolerable degree. When preparing the mother liquor, it is also essential to choose the concentration of mother liquor to reach a certain amount. That is to say, it is to pick up the appropriate amount of solvent for the liquor to make its concentration in accord with the corresponding dose of grading standards to be able to bring its due impact on the acute toxicity. Starting from this basic need to come to the standard, we tried to use the Pentyl xanthate, the ammonium dibuyldithiophosphate, the oxyparaffin soap purified water as solvent at the room temperature conditions. And, later, when the Lauryl amine generated compound with hydrochloric acid, we began to feel it is right moment to try to dissolve GE - 601 by using the diluted hydrochloric acid, the salicylhydroxamic acid, and tried to dissolve the H205 and Z200 by DMSO in high temperature conditions. When this was over, the dose should be chosen and determined to be fit for leading to 0 -100% mortality of the mice, that is, to work out the toxicity of the 6

关 键 词：毒理学 改良寇氏法 最大耐受剂量法 小鼠 急性毒性 LD50 

分 类 号：X503.221[环境科学与工程—环境工程]