拟南芥悬浮细胞中ERG437蛋白的细胞定位的初步观察  

Cellular localization analysis of ERG437 Protein in Arabidopsis Suspension cells

作　　者：杨青青[1] 

机构地区：[1]北京教育学院教师教育数理学院,北京100044

出　　处：《生命科学仪器》2010年第6期46-47,共2页Life Science Instruments

摘　　要：近来在大肠杆菌中新发现的Era蛋白(E.coli ras-like protein)是一类新的GTP结合蛋白,研究表明Era蛋白参与调节细胞分裂、细胞周期以及部分细胞代谢过程。植物中相关研究报道尚少,推测ERG蛋白可能定位在线粒体,并且与种子的正常发育相关。本实验通过构建植物表达载体初步观察了ERG437蛋白在拟南芥悬浮细胞中的定位情况,同时利用CoxⅣ蛋白初步观察到绝大部分ERG437蛋白定位于线粒体。Recently,Era protein （E.coli ras-like protein）,found in E coli cell,is a new kind of GTP-binding protein. The existing research indicates that the Era protein participates in the cell division,the mitotic cycle as well as some cellular metabolism processes. But the eukaryon plant＇s related research report is still few,suggesting that this protein localizes in mitochondria and will be possible related to the seed＇s normal growth. Through this paper,We has observed the ERG437 protein＇s cell localization in arabidopsis＇s suspension cells and has proven the ERG437 protein locates in the mitochondria by using the CoxIV protein.

关 键 词：ERA蛋白 ERG基因 细胞周期 悬浮细胞 

分 类 号：Q943[生物学—植物学]